Sunita Dutta – författare

The book comprises seven sections, each covering different aspects of laboratory biotechnology: general procedures such as pipetting, solution preparation, and the principles of common analytical instruments; working with nucleic acids, bacteria, enzymes, and proteins; cloning experiments and some protocols on plant biotechnology. Emphasis is placed on DNA/RNA isolation from various sources, the use of restriction enzymes, ligation techniques, cloning protocols, the screening of transformed cells, various electrophoresis techniques, PCR protocols, and more. The appendices in the final section offer additional information that is essential to the study of these practical procedures as a whole. This manual will be useful to students in the fields of biotechnology, agriculture, and related disciplines. The aim of this manual was to provide a theoretical foundation for the practical studies that are conducted in the laboratory, in a clear and understandable manner.

The seven sections of this book cover general laboratory procedures, such as pipetting, solution preparation, and the operation of common analytical instruments, as well as specific techniques for working with nucleic acids, bacteria, enzymes, and proteins. The book also includes information on cloning experiments and plant biotechnology protocols. The text emphasizes the isolation of DNA and RNA from various sources, the use of restriction enzymes, ligation techniques, cloning protocols, and the screening of transformed cells. Additionally, it covers a range of electrophoresis techniques, PCR protocols, and other essential laboratory techniques.The appendices at the end of the book provide supplementary information that is relevant to the study of the practical applications covered throughout the manual. This book will be of particular use to students in the fields of biotechnology, agriculture, and related disciplines. The goal of this practical manual is to provide a clear and comprehensive theoretical foundation for the practical laboratory studies that students will undertake.