Tom Sexton - Böcker

This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.

This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.

This new Handbook of Family Therapy is the culmination of a decade of achievements within the field of family and couples therapy, emerging from and celebrating the dynamic evolution of marriage and family theory, practice, and research. The editors have unified the efforts of the profession's major players in bringing the most up-to-date and innovative information to the forefront of both educational and practice settings. They review the major theoretical approaches and break new ground by identifying and describing the current era of evidence-based models and contemporary areas of application. The Handbook of Family Therapy is a comprehensive, progressive, and skillful presentation of the science and practice of family and couples therapy, and a valuable resource for practitioners and students alike.

Cummiskey Alley brings together the best of Tom Sextons poems about the place where he was born and grew up, the mill-lined river city of Lowell, Massachusetts -- a place he never took his eye off, no matter his location. For most of his life hes been in Alaska, writing, teaching, editing a respected literary journal, and always observing the large and small wonders in the world. Hes filled many books with poems that tell us what hes seen and heard and felt. In the Northwest and around the Pacific Rim, Sexton is known as a premier poet of the natural world, from birds to mountains. But theres another side to this writer, a deep investigator and lyrical beat reporter whose subject is his working-class, ethnic-American hometown where hes returned regularly, sometimes anonymously to better absorb the facts and fill the blotter at the night desk in the hall of records. The poems hes drawn from memory and recent inspection stand for the experience of a thousand small industrial cities that were made by immigrants and often got knocked down by merciless economic winds, only to get their legs back under them and move forward. As universal as they may be, places like Lowell need a literature to call their own. The New York Times described Sexton as an atavistic avatar of how to look hard yet write simply. Merrimack Valley Magazine wrote: Each poem unveils something new, and at times breathtaking, about one of the Merrimack Valleys most diverse and interesting places . . . Sextons characters, relationships, and places spring from the page, brought to life by a tiny gesture or minute detail.