This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.- Includes a glossary of over 200 terms important for understanding molecular biology- Uses an inexpensive source of eukaryotic cells - great for schools on a budget- Includes Methods Locator that provides instant access to the latest methods- Contain clearly written, easy-to-follow, student-tested instructions:- Sterile techniques- Phage titration- Gel electrophoresis of DNA- Restriction enzyme digestion- Plasmid isolation- Transformation of E. Coli- Recombinant DNA cloning- Nick translation labeling- Nonradioactive primer labelling- Nonradioactive DNA detection- Southern blotting- Colony hybridization- Purification of plant DNA- RNA purification- Northern blotting- Purification of poly A+ RNA- Polymerase chain reaction (PCR)
