Molecular Microbial Diagnostic Methods

Dr. Nigel Cook is Senior microbiologist at The Food and Environment Research Agency (FERA) in the UK. He specializes in integrated monitoring and control of foodborne viruses in European Food Supply Chains. Martin D’Agostino has worked for Fera Science Ltd, formerly the Food and Environment Research Agency (Fera) since 1994. His microbiological career began with Kennerty Farm Dairies in Aberdeen (now Robert Wisemans) as a laboratory assistant, until he joined what was the Torry Food Science Laboratory (Central Science Laboratory, MAFF) in 1994, now named the Food and Environment Research Agency as part of Defra. He has been involved in EU Framework 5, 6 and 7 projects dealing with such areas as Food PCR, Surveillance of Adenovirus and Norovirus in European Bathing waters and more recently has assisted in the coordination of the FP7 project entitled “Integrated monitoring and Control of Foodborne Viruses in European Food Supply Chains” (www.eurovital.org). This has resulted in the accumulation of many European and worldwide contacts from both Academia and Industry related to both food and environmental microbiology. Martin has experience of chairing and presenting scientific talks at international scientific meetings and has been an author in at least 25 peer reviewed articles including journal manuscripts, several reviews and book chapters and is an editor of a book, related to the detection and implementation of rapid methods for foodborne and environmental human pathogens. Dr. K. Clive Thompson is Fellow of the Royal Society of Chemistry; Fellow of the Institute of Food Science & Technology; Fellow of the Royal Society of Public Health; Member of the Water Management Society; Member of the American Chemical Society; Member of the American Society for Microbiology; Fellow of the Chartered Institute of Water and Environmental Management; Member of Board of Trustees of Society of Chemical Industry; Society of Chemical Industry (SCI) Environmental Medal (2003); Member of AOAC; Member of the Society of Environmental Toxicology and Chemistry; Distinguished Service Certificate, British Standards in appreciation of long and valued contributions to the development of British, European and International Standards; and Visiting Professor at Brunel University.He is currently Chief Scientist at ALcontrol Laboratories UK, which analyses a very wide range of samples including food, drinking water, process waters, and effluents for both chemical and microbiological parameters. It has eight laboratories in the UK and is one of the largest contract contaminated land analysis / water analysis / food analysis laboratory organisations in Europe. Dr Thompson previously worked for Yorkshire water and Severn Trent Water. He is a member of many national and international standardisation committees and has published more than 40 peer-reviewed papers and co-edited a significant number of books.

• 1. Food industry current status2. Future directions for molecular microbial diagnostic methods for the food industry3. Current status of molecular microbiological techniques for the analysis of drinking water4. Future directions for molecular microbial diagnostic methods for the water industry  5. EN ISO Standards for both culture and molecular methods6. Laboratory validation, verification and accreditation of molecular methods 7. DNA extraction: finding the most suitable method8. Assessing organism viability and interpreting genomic unit vs. colony forming unit data for water and food borne microorganisms such as Legionella, Campylobacter, Salmonella and Listeria9. MALDI-TOF: A rapid microbiological confirmation technique for food and water analysis10. Chapter highlights, future requirements and conclusions