Membrane Proteins - Production and Functional Characterization

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

• Section I: Recombinant Expression of Membrane Proteins1. Engineering Escherichia Coli for Functional Expression of Membrane Proteins Franz Y Ho and Bert Poolman2. ACEMBLing a Multiprotein Transmembrane Complex:  The Functional SecYEG-SecDFYajC-YidC  Holotranslocon Protein Secretase/InsertaseJoanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel and Imre Berger3.  Expression and Purification of OsVDAC4Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy and M. K. Mathew4. Membrane Protein Expression in Lactococcus LactisMartin S. King, Christoph Boes and Edmund R.S. Kunji5. An Unconventional Anaerobic Membrane Protein Production System Based on Wolinella SuccinogenesMichael Lafontaine and C. Roy D. Lancaster6. Membrane Protein Expression and Analysis in YeastKatrien Claes, Mouna Guerfal and Nico Callewaert7. Heterologous Expression of G Protein Coupled Receptors in YeastNicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette Byrne8. Recombinant G protein-Coupled Receptor Expression in Saccharomyces Cerevisiae for Protein CharacterizationKory M. Blocker, Zachary T. Britton, Andrea N. Naranjo, Patrick M. McNeely, Carissa L. Young and Anne S. Robinson9. Baculovirus Mediated Expression of GPCRs in Insect CellsTuulia Saarenpää, Veli-Pekka Jaakola and Adrian Goldman10. Expression of Membrane Proteins in the Eyes of Transgenic Drosophila Melanogaster Yvonne Hackmann, Lisa Joedicke, Valérie Panneels and Irmgard Sinning11. Rapid Method to Express and Purify Human Membrane Protein Using the Xenopus Oocyte System for Functional and Low-Resolution Structural AnalysisBenjamin Clémençon, Michael Fine, Philipp Schneider and Matthias A. Hediger12. Expression of G protein-coupled Receptors in Mammalian CellsRaja Chakraborty, Bing Xu, Rajinder P. Bhullar and Prashen Chelikani13. Construction of Stable Mammalian Cell Lines for Inducible Expression of G Protein-Coupled Receptors Chikwado A. Opefi, Dale Tranter, Steven O. Smith and Philip J. Reeves.14. Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI– Cells Using a PiggyBac Inducible SystemLydia N. Caro, Zhijie Li, James M. Rini, Aidin R. Balo and Oliver P. Ernst15. Semliki Forest Virus-based Expression of Recombinant GPCRsKenneth Lundstrom16. Screening for Lipid Requirements of Membrane Proteins by Combining Cell-Free Expression with NanodiscsErik Henrich, Volker Dötsch, and Frank BernhardSection II. Reconstitution and Functional Characterization of Membrane Proteins17. Liposome Reconstitution and Transport Assay for Recombinant TransportersZachary Lee Johnson and Seok-Yong Lee18. Lipid Reconstitution and Recording of Recombinant Ion ChannelsRandy Stockbridge and Ming-Feng Tsai19. Reconstitution of Membrane Proteins - a GPCR as an ExampleAlan D. Goddard, Patricia M. Dijkman, Roslin J. Adamson, Rosana Inácio dos Reis and Anthony Watts20. Ion Channel Reporter for Monitoring the Activity of Engineered GPCRsChristophe J. Moreau,  Katarzyna Niescierowicz , Lydia Caro , Jean Revilloud and  Michel Vivaudou21. Methods for Labeling Skeletal Muscle Ion Channels Site-Specifically With Fluorophores Suitable for FRET-based Structural AnalysisMohana Mahalingam and James D. Fessenden22. Structural and Functional Studies of Nirc From Salmonella TyphimuriumAdriana Rycovska-Blume,Wei Lü, Susana Andrade, Klaus Fendler and Oliver Einsle23. Surface Plasmon Resonance Analysis of 7-Transmembrane Receptors Tonia Aristotelous, Andrew L. Hopkins and Iva Navratilova24. Crosslinking Strategies to Study Peptide Ligand-Receptor Interactions Jeffery Becker25. From recombinant expression to crystals – A step-by-step guide to GPCR crystallographyArun K. Shukla, Punita Kumari, Eshan Ghosh and Kumari Nidhi26. Structure-based Biophysical Analysis of the Interaction of Rhodopsin with G Protein and ArrestinMartha E. Sommer, Matthias Elgeti, Peter W. Hildebrand, Michal Szczepek, Klaus Peter Hofmann and Patrick Scheerer