Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome SamplesA. Otto, S. Maaß, F. Bonn, K. Büttner and D. Becher2. Filter Aided Sample Preparation the Versatile and Efficient Method for Proteomic AnalysisJ.R. Wiśniewski3. An Overview of Advanced SILAC Labeling Strategies for Quantitative ProteomicsF. Terzi and S. Cambridge4. Label Free and Standard Free Absolute Quantitative Proteomics Using the ‘Total Protein’ and ‘Proteomic Ruler’ ApproachesJ.R. Wiśniewski5. Separation of Two Distinct O-Glycoforms of Human IgA1 by Serial Lectin-Chromatography Followed by Mass Spectrometry O-Glycan AnalysisS. Lehoux and T. Ju6. Monitoring Protein Synthesis in Caenorhabditis Elegans Using SILACM. Miyagi7. Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content AnalysisK.D. Ha, S.M. Bidlingmaier, Y. Su, N.-K. Lee and B. Liu8. Beyond the Natural Proteome: Non-Degenerate Saturation Mutagenesis - Methodologies and AdvantagesM.M. Ferreira Amaral, L. Frigotto and A.V. Hine9. WGCNA Application to Proteomic and Metabolomic Data AnalysisG. Pei, L. Chen and W. Zhang10. A Protocol for Large-Scale Proteomic Analysis of Microdissected Formalin Fixed and Paraffin Embedded TissueP. Ostasiewicz and J.R. Wiśniewski11. Body Fluid Degradomics and Characterization of Basic N-TerminomeF. Sabino, O. Hermes and U. auf dem Keller12. Reannotation of Genomes by Means of Proteomics DataJ. Armengaud13. Proteogenomics: Recycling Public Data to Improve Genome AnnotationsA. McAfee and L.J. Foster14. Targeted in-Depth Quantification of Signaling Using Label-Free Mass SpectrometryP.R. Cutillas15. Biotin Switch Assays for Quantitation of Reversible Cysteine OxidationR. Li and J. Kast16. Application of a Thioredoxin Trapping Mutant for Analysis of the Cellular NitrosoproteomeM. Benhar17. Combining Click-Chemistry Based Proteomics with Dox-Inducible Gene ExpressionJ. Gebert, M. Schnölzer, U. Warnken and J. Kopitz18. Analysis of the Proteome of Hair-Cell Stereocilia by Mass SpectrometryJ.F. Krey, P.A. Wilmarth, L.L. David and P.G. Barr-Gillespie19. Proteomic Analysis of Metabolic Responses to Biofuels and Chemicals in Photosynthetic CyanobacteriaT. Sun, L. Chen and W. Zhang20. Deep Profiling of Proteome and Phosphoproteome by Isobaric Labeling, Extensive Liquid Chromatography and Mass SpectrometryB. Bai, H. Tan, V.R. Pagala, A.A. High, V.P. Ichhaporia, L. Hendershot and J. Peng21. Glycoprotein Enrichment Analytical Techniques: Advantages and DisadvantagesR. Zhu, L. Zacharias, K.M. Wooding, W. Peng and Y. Mechref22. Quantitative Glycomics: A Combined Analytical and Bioinformatics ApproachL. Veillon, S. Zhou and Y. Mechref

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome SamplesA. Otto, S. Maaß, F. Bonn, K. Büttner and D. Becher2. Filter Aided Sample Preparation the Versatile and Efficient Method for Proteomic AnalysisJ.R. Wiśniewski3. An Overview of Advanced SILAC Labeling Strategies for Quantitative ProteomicsF. Terzi and S. Cambridge4. Label Free and Standard Free Absolute Quantitative Proteomics Using the ‘Total Protein’ and ‘Proteomic Ruler’ ApproachesJ.R. Wiśniewski5. Separation of Two Distinct O-Glycoforms of Human IgA1 by Serial Lectin-Chromatography Followed by Mass Spectrometry O-Glycan AnalysisS. Lehoux and T. Ju6. Monitoring Protein Synthesis in Caenorhabditis Elegans Using SILACM. Miyagi7. Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content AnalysisK.D. Ha, S.M. Bidlingmaier, Y. Su, N.-K. Lee and B. Liu8. Beyond the Natural Proteome: Non-Degenerate Saturation Mutagenesis - Methodologies and AdvantagesM.M. Ferreira Amaral, L. Frigotto and A.V. Hine9. WGCNA Application to Proteomic and Metabolomic Data AnalysisG. Pei, L. Chen and W. Zhang10. A Protocol for Large-Scale Proteomic Analysis of Microdissected Formalin Fixed and Paraffin Embedded TissueP. Ostasiewicz and J.R. Wiśniewski11. Body Fluid Degradomics and Characterization of Basic N-TerminomeF. Sabino, O. Hermes and U. auf dem Keller12. Reannotation of Genomes by Means of Proteomics DataJ. Armengaud13. Proteogenomics: Recycling Public Data to Improve Genome AnnotationsA. McAfee and L.J. Foster14. Targeted in-Depth Quantification of Signaling Using Label-Free Mass SpectrometryP.R. Cutillas15. Biotin Switch Assays for Quantitation of Reversible Cysteine OxidationR. Li and J. Kast16. Application of a Thioredoxin Trapping Mutant for Analysis of the Cellular NitrosoproteomeM. Benhar17. Combining Click-Chemistry Based Proteomics with Dox-Inducible Gene ExpressionJ. Gebert, M. Schnölzer, U. Warnken and J. Kopitz18. Analysis of the Proteome of Hair-Cell Stereocilia by Mass SpectrometryJ.F. Krey, P.A. Wilmarth, L.L. David and P.G. Barr-Gillespie19. Proteomic Analysis of Metabolic Responses to Biofuels and Chemicals in Photosynthetic CyanobacteriaT. Sun, L. Chen and W. Zhang20. Deep Profiling of Proteome and Phosphoproteome by Isobaric Labeling, Extensive Liquid Chromatography and Mass SpectrometryB. Bai, H. Tan, V.R. Pagala, A.A. High, V.P. Ichhaporia, L. Hendershot and J. Peng21. Glycoprotein Enrichment Analytical Techniques: Advantages and DisadvantagesR. Zhu, L. Zacharias, K.M. Wooding, W. Peng and Y. Mechref22. Quantitative Glycomics: A Combined Analytical and Bioinformatics ApproachL. Veillon, S. Zhou and Y. Mechref