Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

David Chenoweth is at University of Pennsylvania, PA, USA

• Fluorogenic detection of protein aggregates in live cells using the AggTag methodKwan Ho Jung and Xin ZhangSynthesis and application of a ratiometric probe for hydrogen peroxideRuwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. StainsProtein labeling for FRET with methoxycoumarin and acridonylalanineChloe M. Jones, Yarra Venkatesh and E. James PeterssonAscertaining the activity and inhibition of adenosine deaminase via fluorescence-based assaysPaul T. Ludford III and Yitzhak TorMethod to discriminate amyloids using fluorescent probesKristine L. Teppang, Rachel S. Ehrlich and Jerry YangChemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. FarkasMethods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancerJohn C. Widen, Martina Tholen, Joshua J. Yim and Matthew BogyoAcid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organellesNanxi Wang and Lei Wang4-Cyanoindole-based fluorophores for biological spectroscopy and microscopyArusha Acharyya, Ismail A. Ahmed and Feng GaiDissecting contributions of catalytic and reader domains in regulation of histone demethylationNektaria Petronikolou, James E. Longbotham and Danica Galonić FujimoriOrtho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclizationQing Zhang, Yue Zhang and Xuechen LiChemical synthesis of di-ubiquitin modified histones for further biochemical studiesJia-Bin Li, Jun Liang and Changlin TianChemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignanciesSwathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela ChiosisFlICk (fluorescent isoindole crosslinking) for peptide staplingMihajlo Todorovic and David M. PerrinSelective protein N-terminal labeling with N-hydroxysuccinimide estersHanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. DempseyCovalent live-cell labeling of proteins using a photoreactive fluorogenTewoderos M. Ayele, Steve D. Knutson and Jennifer M. HeemstraPhotoconvertible diazaxanthilidene dyes for live cell imagingRachel M. Lackner, Christopher L. Johnny and David M. ChenowethNo-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag Wei Sheng, James H. Geiger and Babak Borhan