Genome Engineering

Dr Gurtler completed a Ph.D. program in 1996 at La Trobe University in the Department of Microbiology. The main contribution of this work was the development of a universal bacterial typing technique based on the 16S-23S rDNA spacer region. The technique is now extensively used in many areas of Microbiology including Diagnostic, Environmental and Veterinary Microbiology. Citations to the articles Dr Gurtler wrote on this topic total >1000 with >830 citations alone to the 1996 review published in the journal “Microbiology”. Dr Gurtler wrote an invited review on genomic typing, taxonomy and identification of bacterial isolates for the International Journal of Systematic and Evolutionary Microbiology (IJSEM). In addition to fundamental research, Dr Gurtler has also had experience with the application of this technology to the medical diagnostic laboratory in the fields of molecular microbiology and human molecular genetics. Dr Gurtler has been involved full time in diagnostic microbiology over many years resulting in the adoption of a method that identifies Mycobacterium species directly from clinical specimens without the need for culture in specimens positive for acid fast bacilli, the discovery of Nocardia veterana, and the development of many diagnostic tests.In the last twelve years Dr Gurtler has been an Editor for the Journal of Microbiological Methods (JMM) and in the last 5 years Serial Editor of Methods in Microbiology (MIM) with the publication of 9 volumes covering diverse subjects such as Biofilms, Nanotechnology, COVID-19 and Fluorescent Probes.Dr. Michael (Mick) Calcutt is a Professor and the Director of Graduate Studies for the Molecular Pathogenesis and Therapeutics Program at the Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri. He completed his BSc. in the University of Sheffield, UK, and his Ph.D in the University of Leicester, UK.Dr. Calcutt's research focuses on aspects of the molecular genetics and pathogenesis of Mycoplasmas, a diverse genus of cell wall-less bacteria that includes many important pathogens of food animals and humans. In the absence of a cell wall, the single limiting membrane represents the unique and critical interface between the pathogen and host. Analysis of the repertoire, function and potential antigenic variation of surface membrane proteins is one of the primary research interests in the laboratory, together with understanding chromosomal dynamics and the role of mobile genetic elements in such plasticity. Increasingly, whole genome sequencing and comparative genomic approaches are being exploited to systematically assess the deduced surface proteome, horizontal gene transfer and accessory gene pool that likely contribute to the pathobiology of these organisms.

• 1. Whole genome transplantation in MollicutesJörg Jores, Fabien Labroussaa and Sergi Torres Puig2. Natural transformation as a tool in Acinetobacter baylyi: evolution by amplification of gene copy numberEllen L. Neidle, Suvi Santala and Isabel Pardo Mendoza3. Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysisSuvi Santala, Ellen L. Neidle and Isabel Pardo Mendoza4. Methods to characterize cryptic biosynthetic gene clusters in StreptomycesChung Thanh Nguyen5. Genome engineering in bacteria: current and prospective applicationsRuben Dario Arroyo Olarte, Edgar Morales Ríos and Karla Daniela Rodríguez Hernández6. Tools for activation and identification of cryptic biosynthesis pathways in StreptomycesHue Nguyen