Biological NMR Spectroscopy

"a useful collection. . . . The book may serve as an effective reference work with a potpourri of collected papers. . . . a well-referenced and handy text with and excellent overview of biological NMR spectroscopy."--Concepts in Magnetic Resonance"Excellent. . . .This text should be required reading for all scientists interested in, or involved with, the application of NMR to biological problems. The text provides not only a clear overview of the state of the art in the field but also, in my opinion, an equally important overview of the history of the field."--Journal of Medicinal Chemistry"Nuclear magnetic resonance spectroscopy is an extremely powerful tool in characterizing a variety of biological problems, ranging from protein structure determination, to characterization of enzyme reaction mechanisms, to in vivo metabolism and medical imaging. . . .Overall, the book is quite enjoyable and it essentially accomplishes its aim to provide 'a contemporary overview of the legacy of these early (NMR) experiments'. . .in modern structuralbiology." --Applied Spectroscopy"The contributors survey the historical development and roles of NMR in a group of introductory essays, and further explore the contemporary research being done in the field with specific discussions."--SciTech Book News"a useful collection. . . . The book may serve as an effective reference work with a potpourri of collected papers. . . . a well-referenced and handy text with and excellent overview of biological NMR spectroscopy."--Concepts in Magnetic Resonance"Excellent. . . .This text should be required reading for all scientists interested in, or involved with, the application of NMR to biological problems. The text provides not only a clear overview of the state of the art in the field but also, in my opinion, an equally important overview of the history of the field."--Journal of Medicinal Chemistry"Nuclear magnetic resonance spectroscopy is an extremely powerful tool in characterizing a variety of biological problems, ranging from protein structure determination, to characterization of enzyme reaction mechanisms, to in vivo metabolism and medical imaging. . . .Overall, the book is quite enjoyable and it essentially accomplishes its aim to provide 'a contemporary overview of the legacy of these early (NMR) experiments'. . .in modern structuralbiology." --Applied Spectroscopy"The contributors survey the historical development and roles of NMR in a group of introductory essays, and further explore the contemporary research being done in the field with specific discussions."--SciTech Book News

• SECTION I: HISTORY OF THE BIOLOGICAL NMR SPECTROSCOPY ; 1. Simple Insights from the Beginnings of Magnetic Resonance in Molecular Biology, ; 2. Choice of Problems in the Early Days of Biochemical NMR, ; 3. Early Days of Biochemical NMR, ; 4. William D. Phillips Memorial Lecture, ; SECTION II: PROTEIN STRUCTURAL STUDIES ; 5. Flexibility & Function of the Escherichia coli trp Repressor, ; 6. Heteronuclear Strategies for the Assignment of Larger Protein/DNA Complexes, ; 7. Design of Novel Hemoglobins, ; 8. The Role of NMR Spectroscopy in Understanding how Proteins Fold, ; 9. NMR Approaches to Understanding Protein Specificity, ; 10. NMR and Mutagenesis Investigations of a Model Cis:Trans Peptide Isomerization Reaction, ; 11. A Solid State NMR Approach to Structure Determination of Membrane Associated Peptides and Proteins, ; 12. NMR Approach to the Heat-, Cold-, and pressure-Induced Unfolding of Proteins, ; 13. Multidimensional NMR Investigation of the Neurotoxic Peptide mastoparan in the Absence and Presence of Calmodulin, ; 14. NMR of Larger Proteins, ; 15. Selective Chemical Deuteration of Aromatic Amino Acids, ; 16. The Interactions of Antigens and Superantigens with the Human Class II Major Histocompatibility complex Molecule HLA-DR1, ; 17. Chorismate Mutase, Essentially a Template Enzyme, ; SECTION III: NUCLEIC ACIDS ; 18. Computing the Structure of Large Complexes, ; 19. Design and Characterization of New Sequence Specific DNA Ligands, ; 20. Determination by 1H NMR of a Slow Conformational Transition and Hydration Change in the Consensus TATAAT Pribnow Box, ; SECTION IV: IN VIVO SPECTROSCOPY ; 21. Clinicians Need Localized Proton MRS of the Brain in the Management of HIV-related Encephalopathies, ; 22. Nuclear Magnetic Resonance Spectroscopy Studies of cancer Cell Metabolism, ; 23. Ex Vivo Multinuclear NMR Spectroscopy of Perfused respiring Rat Brain Slices,