Antibody Engineering

A Practical Approach

AvHoogenboom McCafferty,John McCafferty

Inbunden, Engelska, 1996

Del 169 i serien Practical Approach Series

1 758 kr

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Beskrivning

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

Produktinformation

Utgivningsdatum:1996-06-27
Mått:155 x 235 x 19 mm
Vikt:522 g
Format:Inbunden
Språk:Engelska
Serie:Practical Approach Series
Antal sidor:348
Förlag:OUP OXFORD
ISBN:9780199635924

Utforska kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Recensioner i media

This book is very useful for practising antibody engineers, providing extra information that enables them to adopt new techniques or expand into new areas. ... this is a useful and interesting resource for practising antibody engineers and I will make sure my students have access to it.

Innehållsförteckning

1. Construction and use of antibody gene repertoires ; 2. Affinity maturation of antibodies using phage display ; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs ; 4. Measuring antibody affinities in solution ; 5. Measuring antibody affinities using Bio-sensors ; 6. Analysis of human antibody sequences ; 7. Rodent to human antibodies by CDR grafting ; 8. Rodent to human antibodies by phage display ; 9. Choosing and manipulating effector functions ; 10. Secretion and purification of antibody fragments expressed in bacteria ; 11. Production of single chain Fv monomers and multimers ; 12. Expression of immunoglobulin genes in mammalian cells ; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms