Extracellular Vesicles for Therapeutic and Diagnostic Applications

Anand Krishnan is Principal Investigator in Chemical Pathology at the Faculty of Health Sciences, University of the Free State, South Africa, where he leads the Precision Medicine Integrative Nano Diagnostics (P-MIND) laboratories. His research focuses on the biological functions of extracellular vesicles in synthetic biology, including cell surface protein modification, biomarker discovery using liquid biopsy-derived extracellular vesicles, and the role of circulating biomarkers in disease development and stem cell biology. He leads multidisciplinary research in precision medicine and nanoscale diagnostics, with a particular interest in translational applications of extracellular vesicle science. Krishnan is an active member of the International Society for Extracellular Vesicles, the African Organisation for Research and Training in Cancer, and the American Association for Cancer Research. He has also served in editorial roles supporting scientific research and publishing. Dr. Vadivalagan Chithravel works as a Research Fellow in the Department of Surgery at the University of Michigan, Ann Arbor, specializing in medical science, excluding epidemiology. The Exosome Mediated Signal Transduction Mechanism is his area of expertise. His current work focuses on a glycosylation biomarker associated with an exosome pathway for the physiology of cells with HCC. In addition to being a group director and scientist at the SAARP foundation in India, he has held postdoctoral research fellow positions at Taipei Medical University and China Medical University in Taiwan. He is also an active member of the International Society of Extracellular Vesicles and the American Association of Extracellular Vesicles. Prakash Gangadaran is Visiting Research Professor and Principal Investigator in the Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Republic of Korea. His research focuses on extracellular vesicles and their applications in angiogenesis, wound healing, drug delivery, hair growth, osteoarthritis, and molecular and nuclear imaging. He has led externally funded research projects in extracellular vesicle biology and is actively involved in advancing their translational and therapeutic applications. In addition to his research activities, he is a member of the International Society for Extracellular Vesicles and the Korea Society of Extracellular Vesicles. His work spans the development and evaluation of extracellular vesicle-based approaches for regenerative medicine, imaging, and targeted therapies. Dr. Sathish Muthu is an Assistant Professor in the Department of Orthopaedics at Government Medical College, Karur, India. He is the Co-Founder and Head of Research at Orthopaedic Research Group, a non-profit research organization dedicated to translational orthopaedic research. He is also a steering committee member of the Indian Stem Cell Study Group. and associate member of Knowledge Forum Degenerative of AO Spine. Despite being a board-certified orthopaedic surgeon, Dr. Muthu holds fellowships in spine surgery, orthopaedic rheumatology, and regenerative medicine. He has published over 275 articles in international peer-reviewed journals and has contributed 5 chapters to books on cartilage injury and regeneration. He is also an Associate Editor at various academic journals like PLOS One, Journal of Clinical Medicine, Indian Journal of Orthopaedics, Journal of Orthopaedic Case Reports. His research focuses on cartilage biology, mesenchymal stem cells, exosomes, degenerative disorders, osteoarthritis, and cartilage regeneration. Dr. Ben Peacock is Head of Research at NanoFCM Co., Ltd. He has expertise in exosomal miRNA and protein profiles of EVs from cancer cell lines by RNA-seq and mass spectrometry (ion-trap) with validation by RT-qPCR and further western blotting. In international peer-reviewed journals, he has published more than nine research articles. His research interests include EV research, but he is well established in stromal communication of cancers of the head and neck. Multiple procedures were optimized to maximize EV generation from cell lines, improve concentration and purity of EV isolations, and implement best practices for sample storage.

• Isolation and purification of exosome and other extracellular vesiclesOverview of the characterization of extracellular vesiclesExtracellular vesicles for therapeutic and diagnostic applicationsPreclinical evidence of extracellular vesicles for therapeutic and diagnostic applicationsExtracellular vesicles as biomarker and therapeutic targets for neurological disordersRevolutionizing cardiovascular health: exploring the extracellular vesiclesExtracellular vesicles for infectious diseases: a game-changer in infectious disease controlExtracellular vesicles against viral pathogens and infectious diseasesTheranostic potential of extracellular vesicles released from mycobacteria and mycobacterium-infected host cellsExtracellular vesicles in reproductive medicinesExtracellular vesicles for ophthalmic and dermal ailmentsExtracellular vesicles for intervertebral disc regenerationExtracellular vesicles: unlocking therapeutic potential in regenerative medicineRole of extracellular vesicles in neurodegenerative diseasesExtracellular vesicle: the future personalized, targeted drug therapy towards cancer managementExosome and other extracellular vesicles for bone diseasesIn silico techniques, artificial intelligence, and machine learning for enhanced efficacy of extracellular vesicle-based diagnosis and therapeuticsRegulatory concerns for exosome- and other extracellular vesicle-based diagnostics and medicine products