Krishnarao Appasani is the Founder and Chief Executive Officer of Gene Expression Systems, a gene discovery company, focusing on functional genomics in cancer research. He is the Editor of RNA Interference: From Basic Science to Drug Development (2005).

'… comprehensive and detailed … compiled logically … informative and easy to read.' Microbiology Today

• Foreword; Foreword; Introduction Part I. Discovery of MicroRNAs in Various Organisms: 1. The microRNAs of C. elegans; 2. Non-coding RNAs - development of man-made vector-based intronic microRNAs; 3. Seeing is believing: strategies for studying microRNA expression; 4. MicroRNAs in limb development; 5. Identification of miRNAs in plant oryza sativa; Part II. MicroRNA Functions and RNAi-Mediated Pathways: 6. Inhibition of translation initiation by a microRNA; 7. In situ analysis of microRNA expression during vertebrate development; 8. MicroRNA function in the nervous system; 9. Micro RNA expression that controls the amount of branched chain α-ketoacid dehydrogenase in mitochondria of human cells; 10. MicroRNAs and the regulation of leaf shape; Part III. Computational Biology of MicroRNAs: 11. MiRBase: a database of microRNA sequences, targets and nomenclature; 12. Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes; 13. Computational approaches to elucidate miRNA biology; 14. The RNA hybrid approach to microRNA target prediction; 15. Machine learning predicts microRNA target sites; 16. Models of microRNA-target coordination; Part IV. Detection and Quantitation of MicroRNAs: 17. Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes; 18. Detection and quantitation of microRNAs using the RNA Invader® assay; 19. A single molecule method to quantify miRNA gene expression; 20. Real-time quantification of MicroRNAs by TaqMan® assays; 21. Real-time quantification of miRNAs and mRNAs employing universal reverse transcription; Part V. MicroRNAs in Disease Biology: 22. Dysregulation of microRNAs in human malignancy; 23. High throughput microRNAs profiling in cancers; 24. Roles of microRNAs in cancer and development; 25. MiR-122 in mammalian liver; 26. MiRNAs in glioblastoma; 27. Role of microRNA pathway in Fragile X mental retardation; 28. Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia; 29. MiRNAs in TPA-induced differentiation of HL-60 cells; 30. MiRNAs in skeletal muscle differentiation; 31. Identification and potential function of viral microRNAs; 32. Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase; Part VI. MicroRNAs in Stem Cell Development: 33. MicroRNAs in the stem cells of the mouse Blastocyst; 34. The role of miRNA in hematopoiesis; 35. MicroRNAs in embryonic stem cell differentiation and prediction of their targets; 36. Generation of single cell microRNA expression profile.

Krishnarao Appasani is the Founder and Chief Executive Officer of Gene Expression Systems, a gene discovery company, focusing on functional genomics in cancer research. He is the Editor of RNA Interference: From Basic Science to Drug Development (2005).

'… comprehensive and detailed … compiled logically … informative and easy to read.' Microbiology Today

• Foreword; Foreword; Introduction Part I. Discovery of MicroRNAs in Various Organisms: 1. The microRNAs of C. elegans; 2. Non-coding RNAs - development of man-made vector-based intronic microRNAs; 3. Seeing is believing: strategies for studying microRNA expression; 4. MicroRNAs in limb development; 5. Identification of miRNAs in plant oryza sativa; Part II. MicroRNA Functions and RNAi-Mediated Pathways: 6. Inhibition of translation initiation by a microRNA; 7. In situ analysis of microRNA expression during vertebrate development; 8. MicroRNA function in the nervous system; 9. Micro RNA expression that controls the amount of branched chain α-ketoacid dehydrogenase in mitochondria of human cells; 10. MicroRNAs and the regulation of leaf shape; Part III. Computational Biology of MicroRNAs: 11. MiRBase: a database of microRNA sequences, targets and nomenclature; 12. Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes; 13. Computational approaches to elucidate miRNA biology; 14. The RNA hybrid approach to microRNA target prediction; 15. Machine learning predicts microRNA target sites; 16. Models of microRNA-target coordination; Part IV. Detection and Quantitation of MicroRNAs: 17. Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes; 18. Detection and quantitation of microRNAs using the RNA Invader® assay; 19. A single molecule method to quantify miRNA gene expression; 20. Real-time quantification of MicroRNAs by TaqMan® assays; 21. Real-time quantification of miRNAs and mRNAs employing universal reverse transcription; Part V. MicroRNAs in Disease Biology: 22. Dysregulation of microRNAs in human malignancy; 23. High throughput microRNAs profiling in cancers; 24. Roles of microRNAs in cancer and development; 25. MiR-122 in mammalian liver; 26. MiRNAs in glioblastoma; 27. Role of microRNA pathway in Fragile X mental retardation; 28. Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia; 29. MiRNAs in TPA-induced differentiation of HL-60 cells; 30. MiRNAs in skeletal muscle differentiation; 31. Identification and potential function of viral microRNAs; 32. Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase; Part VI. MicroRNAs in Stem Cell Development: 33. MicroRNAs in the stem cells of the mouse Blastocyst; 34. The role of miRNA in hematopoiesis; 35. MicroRNAs in embryonic stem cell differentiation and prediction of their targets; 36. Generation of single cell microRNA expression profile.

Krishnarao Appasani is the Founder and Chief Executive Officer of Gene Expression Systems, a gene discovery company, focusing on functional genomics in cancer research. He is the Editor of RNA Interference: From Basic Science to Drug Development (2005).

'… comprehensive and detailed … compiled logically … informative and easy to read.' Microbiology Today

• Foreword; Foreword; Introduction Part I. Discovery of MicroRNAs in Various Organisms: 1. The microRNAs of C. elegans; 2. Non-coding RNAs - development of man-made vector-based intronic microRNAs; 3. Seeing is believing: strategies for studying microRNA expression; 4. MicroRNAs in limb development; 5. Identification of miRNAs in plant oryza sativa; Part II. MicroRNA Functions and RNAi-Mediated Pathways: 6. Inhibition of translation initiation by a microRNA; 7. In situ analysis of microRNA expression during vertebrate development; 8. MicroRNA function in the nervous system; 9. Micro RNA expression that controls the amount of branched chain α-ketoacid dehydrogenase in mitochondria of human cells; 10. MicroRNAs and the regulation of leaf shape; Part III. Computational Biology of MicroRNAs: 11. MiRBase: a database of microRNA sequences, targets and nomenclature; 12. Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes; 13. Computational approaches to elucidate miRNA biology; 14. The RNA hybrid approach to microRNA target prediction; 15. Machine learning predicts microRNA target sites; 16. Models of microRNA-target coordination; Part IV. Detection and Quantitation of MicroRNAs: 17. Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes; 18. Detection and quantitation of microRNAs using the RNA Invader® assay; 19. A single molecule method to quantify miRNA gene expression; 20. Real-time quantification of MicroRNAs by TaqMan® assays; 21. Real-time quantification of miRNAs and mRNAs employing universal reverse transcription; Part V. MicroRNAs in Disease Biology: 22. Dysregulation of microRNAs in human malignancy; 23. High throughput microRNAs profiling in cancers; 24. Roles of microRNAs in cancer and development; 25. MiR-122 in mammalian liver; 26. MiRNAs in glioblastoma; 27. Role of microRNA pathway in Fragile X mental retardation; 28. Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia; 29. MiRNAs in TPA-induced differentiation of HL-60 cells; 30. MiRNAs in skeletal muscle differentiation; 31. Identification and potential function of viral microRNAs; 32. Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase; Part VI. MicroRNAs in Stem Cell Development: 33. MicroRNAs in the stem cells of the mouse Blastocyst; 34. The role of miRNA in hematopoiesis; 35. MicroRNAs in embryonic stem cell differentiation and prediction of their targets; 36. Generation of single cell microRNA expression profile.