Manual of Antisense Methodology

AvGunther Hartmann,Stefan Endres

Inbunden, Engelska, 1999

Del i serien Perspectives in Antisense Science

1 589 kr

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Beskrivning

In the late-90s, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense-based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary. This book emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections, antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment. It should be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.

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