Capillary Electrophoresis of Proteins

Inbunden, Engelska, 1998

Del i serien Chromatographic Science Series

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Beskrivning

Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions.

Produktinformation

Utgivningsdatum:1998-10-20
Mått:152 x 229 x 21 mm
Vikt:720 g
Format:Inbunden
Språk:Engelska
Serie:Chromatographic Science Series
Antal sidor:300
Förlag:Taylor & Francis Inc
ISBN:9780824702052

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Analytisk kemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Mer om författaren

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu

Recensioner i media

". . .well written and referenced. It is one of the most comprehensive texts currently available on the application of capillary electrophoresis to protein analysis. . ..a useful source of information for separation scientists and postgraduate students wishing to obtain an overall review of protein capillary electrophoresis separations."---European Journal of Pharmaceutics and Biopharmaceutics

Innehållsförteckning

Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.