Recombinant DNA Principles and Methodologies

Inbunden, Engelska, 1998

5 792 kr

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Beskrivning

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such asplasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Produktinformation

Utgivningsdatum:1998-06-25
Mått:219 x 276 x undefined mm
Vikt:1 570 g
Format:Inbunden
Språk:Engelska
Antal sidor:762
Förlag:Taylor & Francis Inc
ISBN:9780824799892

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Mer om författaren

James Greene (Catholic University, Washington D.C., USA)

Recensioner i media

"…provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits."- Trends in Genetics

Innehållsförteckning

Why Cloning?, James J. GreeneBiochemistry of Nucleic Acids, Paul S. MillerEnzymology of Recombinant DNA, Frank J. CastoraBasic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan GargesFormulation of Cloning Strategies, John B. DameIsolation and Purification of Nucleic Acids, James J. GreeneGel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. CastoraHybridization Probes, Frank J. Castora and James J. GreenecDNA Cloning and the Construction of Recombinant DNA, Dante S. ZarlengaMolecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco BolívarPhage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael FeissCosmid Cloning, John Collins and Werner LindenmaierMultipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. ShortMammalian Expression Vectors, Ricky R. Hirschhorn and Nava SarverApproaches to the Expression of Foreign Genes, Allan R. ShatzmanScreening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. ManakPrinciples and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. DieffenbachDNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. RaoSite-Directed Mutagenesis, Alfred Johnson and Marvin Reitz