DNA Vaccines

"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research "The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc...an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters "Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery "Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses...In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science "The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology "Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today

• Purification of Supercoiled Plasmid.- Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines.- Development and Characterization of Lyophilized DNA Vaccine Formulations.- Repeated Use of Qiagen Columns in Large-Scale Preparation of Plasmid DNA.- The Immunology of DNA Vaccines.- Methodology Used in DNA-Based Prophylactic and Therapeutic Immunization Against Hepatitis B Virus in Chimpanzees.- Intramuscular and Intradermal Injection of DNA Vaccines in Mice and Primates.- Veterinary DNA Vaccines.- DNA-Based Immunization of Neonatal Mice.- Intramuscular Injection of DNA Vaccines in Fish.- Development of DNA Vaccines for Salmonid Fish.- CTL Analysis for Tumor Vaccines.- The Use of Bone Marrow-Chimeric Mice in Determining the MHC Restriction of Epitope-Specific Cytotoxic T Lymphocytes.- Immunostimulatory DNA Sequences.- Immunostimulatory CpG Motifs and DNA Vaccines.- In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors.- Adjuvants for Plasmid DNA Vaccines.- Cytokine and Costimulatory Factor-Encoding Plasmids as Adjuvants for DNA Vaccination.- Optimization of DNA Vaccines Through the Use of Molecular Adjuvants.- Cytokine Fusion Constructs as DNA Vaccines Against Tumors.- The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparations.- Genetic Adjuvants.- DNA Immunization in Combination with the Immunostimulant Monophosphoryl Lipid A.- Controlled Plasmid Delivery and Gene Expression.- Mucosal Immunization with DNA Vaccines.- Preparations for Particle-Mediated Gene Transfer Using the Accell® Gene Gun.- Entrapment of Plasmid DNA Vaccines into Liposomes by Dehydration/Rehydration.- DNA-Based Vaccination with Polycistronic Expression Plasmids.- A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination.-Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES.- Genetic Subunit Vaccines.- Antigen Engineering in DNA Immunization.- Genetic Vaccination Targeting T-Cell Receptors.- Immunity to T-Cell Receptor.- DNA Fusion Vaccines Against B-Cell Tumors.- DNA-Based Vaccination Primes Tumor-Rejecting T-Cell Responses.- Development of Female Contraceptive Vaccine Through DNA Inoculation of Human Chorionic Gonadotropin Beta Subunit (hCGß).- Genetic Immunization for Allergy Immunotherapy.- Preclinical Safety of DNA Vaccines.- DNA Vaccination.- Assuring the Quality, Safety, and Efficacy of DNA Vaccines.