Antibody Phage Display

"...this book lives up to its promises. It starts with an excellent introduction by Hennie Hoogenboom, reprinted from his Immunotechnology article, which sets the scene and provides an overview of the field for new readers...Members of the laboratory that used the book for reference found it very clear and all were keen that the review was completed as quickly as possible so that the book was returned to the laboratory where they could make use of it! I think this in itself supports the fact that the book is a useful volume." - Immunology News "As with other volumes in this popular series, each protocol is discussed in detail, including an introduction, materials, methods notes, and references. It is a good purchase for research libraries where monoclonal antibodies are studied." E-Streams

• Overview of Antibody Phage-Display Technology and Its Applications.- Standard Protocols for the Construction of Fab Libraries.- Standard Protocols for the Construction of scFv Libraries.- Broadening the Impact of Antibody Phage Display Technology.- Construction of Large Naïve Fab Libraries.- Construction of Polyclonal Antibody Libraries Using Phage Display.- Antigen-Driven Stimulation of B-Lymphocytes In Vitro.- 8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion.- Panning of Antibody Phage-Display Libraries.- Selection of Antibodies Against Biotinylated Antigens.- Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries.- Blocking Immunodominant Epitopes by Competitive Deselection.- Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy.- Screening of Phage-Expressed Antibody Libraries by Capture Lift.- Antibody-Guided Selection Using Capture-Sandwich ELISA.- Proximity-Guided (ProxiMol) Antibody Selection.- Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies.- Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques.- Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs.- Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments.- Selection of Antibodies Based on Antibody Kinetic Binding Properties.- Selection of Functional Antibodies on the Basis of Valency.- Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis.- Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement.- Error-Prone Polymerase Chain Reaction for Modification of scFvs.- Use of Escherichia coli Mutator Cells to Mature Antibodies.- ChainShuffling to Modify Properties of Recombinant Immunoglobulins.- Generation of Bispecific and Tandem Diabodies.- High-Level Periplasmic Expression and Purification of scFvs.- Periplasmic Expression and Purification of Recombinant Fabs.- Expression of Antibody Fragments in Pichia pastoris.- Expression of VHH Antibody Fragments in Saccharomyces cerevisiae.- Intrabodies.- 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells.- 35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells.