Ion Channel Localization

"There is a nice balance of review and methodology, with an emphasis on methodological principles. The scope of the book provides a rich and comprehensive review of staid and state-of-the-art localization techniques and their rationales for use. This is a singular and needed addition to the field of ion channel physiology, cell biology and neurobiology"-Doody's Health Sciences Book Review Journal "...both timely and will be of considerable interest to researchers of ion channel function...This book represents a collection of 24 methods of ion channel localization written by leading specialists and divided into four main parts. The first part concentrates on the fluorescent and radioligand binding of channel or receptors with specific pharmacological agents or toxins...Thus, the second part of this book concentrates on the quantification and cellular or subcellular visualization of ion channel expression in oocytes and immortal cell lines using green fluorescent proteins (GFP)...The third section of Ion Channel Localization: Methods and Protocols utilizes the functional properties of the channels for localization...One of the major achievements of Ion Channel Localization: Methods and Protocols in gathering so many of today's technologies of channel visualization is the candid and detailed descriptions the authors have provided of the advantages, pitfalls and 'tricks of the trade' of the methods under discussion. Descriptions are such I am confident that many scientists will be tempted to have a go themselves." - Clinical and Experimental Pharmacology and Physiology

• Pharmacological Labeling of Ion Channels and Receptors.- Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells.- Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies.- GABAA Receptors.- Localization of ATP P2X Receptors.- Small Conductance Calcium-Activated Potassium Channels in Rat Brain.- Mapping N-Type Calcium Channel Distributions with H-Conotoxins.- Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development.- High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels.- Applications of Green Fluorescent Protein (GFP) Technology.- Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells.- Confocal Imaging of GFP-Labeled Ion Channels.- Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes.- Applications of Green Fluorescent Protein (GFP) Technology.- Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models.- Mutant GFP- Based FRET Analysis of K+ Channel Organization.- Delivering Ion Channels to Mammalian Cells by Membrane Fusion.- Functional Assays in Ion Channel Localization.- Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake.- Crystallization Technique for Localizing Ion Channels in Living Cells.- Imaging of Localized Neuronal Calcium Influx.- Using Caged Compounds to Map Functional Neurotransmitter Receptors.- Atomic Force Microscopy.- Atomic Force Microscopy of Reconstituted Ion Channels.- Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy.- Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy.- Dynamic AFM of Patch Clamped Membranes.- Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.