Liposome Methods and Protocols

Inbunden, Engelska, 2002

Del i serien Methods in Molecular Biology

1 119 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Beskrivning

A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid.

Produktinformation

Utgivningsdatum:2002-05-15
Mått:155 x 235 x 21 mm
Vikt:613 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:252
Upplaga:2002
Förlag:Humana Press Inc.
ISBN:9780896038455

Utforska kategorier

Medicinhistoria inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin

Recensioner i media

"...those who deal with liposome in their work, this book with detailed laboratory protocols and many notes will be very useful." -Pharmaceutical Research "Volume 199 of the series Methods in Molecular Biology(TM), offers an excellent overview of protocols used for the study of liposomes in biology...the book portrays comprehensive reports of the study of the cellular fate of sphingolipids using fluoresrole lipids play in virus fusion, studies of functions of various membrane-associated proteins, specifically of phospholipid binding proteins in glycoprotein biosynthesis and contributions of therapeutical liposome applications." -Molecular Biotechnology

Innehållsförteckning

Physical Properties.- Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids.- Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems.- Liposome Fusion/Modulation.- Peptide-Induced Fusion of Liposomes.- Liposomes.- Lipids in Viral Fusion.- Application of Liposomes.- Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells.- Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays.- Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis.- Therapeutic Uses of Antioxidant Liposomes.- Targeted Gene Delivery by Virosomes*.- Liposomes Containing Ligands.- Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes.- Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies.- Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides.- Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins.