Luca Panconi is a mathematician and bioinformatician who specialises in implementing analysis techniques for super-resolution microscopy data. His work crosses the boundaries of computer science and biology, creating innovative data analysis tools that unlock unprecedented details at a molecular level. As a statistician with a commitment to advancing the scientific community, his work bridges the gap between complex mathematics and biological research.Dr. Daniel Nieves has been a research fellow in the lab of Prof. Dylan Owen within the Institute of Immunology and Immunotherapy at the University of Birmingham, UK since 2019. His work has focused on implementation and design of a framework to aid cluster analysis implementation for single molecule localisation microscopy. His research focuses on the development of new labelling and analysis methods to improve quantitative biological measurements using super-resolution microscopy.Dr. Maria Makarova was promoted to Assistant Professor in 2022 in the School of Biosciences. Her work combines comparative microbiology, genetics, lipidomics and advanced microscopy to understand the interplay of metabolism, the membrane and the environment.Prof. Dylan Owen moved to the University of Birmingham in 2019 and now holds an interdisciplinary chair position between the Institute of Immunology and Immunotherapy and the School of Mathematics, as well as serving as the deputy-director of the Centre of Membrane Proteins and Receptors (COMPARE). His lab’s work seeks to develop new microscopy methodology (especially single-molecule localisation microscopy, image analysis and AI) and apply these methods to study membrane biophysics and T cell immunology.

• Chapter 1. General introduction to imaging the Plasma membraneChapter 2. How to map membrane proteins at the single-molecule levelChapter 3. Measuring diffusion of membrane biomolecules using fluorescence microscopyChapter 4. Detecting membrane protein dimers and oligomers by FRETChapter 5. Imaging membrane biophysical propertiesChapter 6. Conclusions and future perspectives

Luca Panconi is a mathematician and bioinformatician who specialises in implementing analysis techniques for super-resolution microscopy data. His work crosses the boundaries of computer science and biology, creating innovative data analysis tools that unlock unprecedented details at a molecular level. As a statistician with a commitment to advancing the scientific community, his work bridges the gap between complex mathematics and biological research.Dr. Daniel Nieves has been a research fellow in the lab of Prof. Dylan Owen within the Institute of Immunology and Immunotherapy at the University of Birmingham, UK since 2019. His work has focused on implementation and design of a framework to aid cluster analysis implementation for single molecule localisation microscopy. His research focuses on the development of new labelling and analysis methods to improve quantitative biological measurements using super-resolution microscopy.Dr. Maria Makarova was promoted to Assistant Professor in 2022 in the School of Biosciences. Her work combines comparative microbiology, genetics, lipidomics and advanced microscopy to understand the interplay of metabolism, the membrane and the environment.Prof. Dylan Owen moved to the University of Birmingham in 2019 and now holds an interdisciplinary chair position between the Institute of Immunology and Immunotherapy and the School of Mathematics, as well as serving as the deputy-director of the Centre of Membrane Proteins and Receptors (COMPARE). His lab’s work seeks to develop new microscopy methodology (especially single-molecule localisation microscopy, image analysis and AI) and apply these methods to study membrane biophysics and T cell immunology.

• Chapter 1. General introduction to imaging the Plasma membraneChapter 2. How to map membrane proteins at the single-molecule levelChapter 3. Measuring diffusion of membrane biomolecules using fluorescence microscopyChapter 4. Detecting membrane protein dimers and oligomers by FRETChapter 5. Imaging membrane biophysical propertiesChapter 6. Conclusions and future perspectives

Luca Panconi is a mathematician and bioinformatician who specialises in implementing analysis techniques for super-resolution microscopy data. His work crosses the boundaries of computer science and biology, creating innovative data analysis tools that unlock unprecedented details at a molecular level. As a statistician with a commitment to advancing the scientific community, his work bridges the gap between complex mathematics and biological research.Dr. Daniel Nieves has been a research fellow in the lab of Prof. Dylan Owen within the Institute of Immunology and Immunotherapy at the University of Birmingham, UK since 2019. His work has focused on implementation and design of a framework to aid cluster analysis implementation for single molecule localisation microscopy. His research focuses on the development of new labelling and analysis methods to improve quantitative biological measurements using super-resolution microscopy.Dr. Maria Makarova was promoted to Assistant Professor in 2022 in the School of Biosciences. Her work combines comparative microbiology, genetics, lipidomics and advanced microscopy to understand the interplay of metabolism, the membrane and the environment.Prof. Dylan Owen moved to the University of Birmingham in 2019 and now holds an interdisciplinary chair position between the Institute of Immunology and Immunotherapy and the School of Mathematics, as well as serving as the deputy-director of the Centre of Membrane Proteins and Receptors (COMPARE). His lab’s work seeks to develop new microscopy methodology (especially single-molecule localisation microscopy, image analysis and AI) and apply these methods to study membrane biophysics and T cell immunology.

• Chapter 1. General introduction to imaging the Plasma membraneChapter 2. How to map membrane proteins at the single-molecule levelChapter 3. Measuring diffusion of membrane biomolecules using fluorescence microscopyChapter 4. Detecting membrane protein dimers and oligomers by FRETChapter 5. Imaging membrane biophysical propertiesChapter 6. Conclusions and future perspectives