Abdul Basit is a Ph.D. researcher at the Department of Horticultural Science, Kyungpook National University, South Korea. He previously worked at the National Agricultural Research Center, Islamabad, and the Climate Change Center, The University of Agriculture, Peshawar. Supported by Pakistani and Korean government scholarships, he has published 67+ articles and 17+ book chapters. He has participated in over 35 scientific events. His research focuses on molecular cytogenetics, plant genomics, breeding, stress physiology, and horticultural crop production.Heba I. Mohamed is a Professor of Plant Physiology at Ain Shams University. Her research focuses on plant stress physiology, biochemistry, eco-friendly stress alleviation, and genetic diversity. She has edited nine books published by international publishers and has also published 40 book chapters, eight reviews, and over 140 research articles. She obtained Encouragement State Award in Basic Sciences (Biological Sciences) 2018 and has been listed among Stanford–Elsevier’s top 2% most influential scientists (2021–2024).Syed Tanveer Shah is an Assistant Professor at the Department of Agriculture, Hazara University, and previously served over a decade in Horticulture at The University of Agriculture, Peshawar. He holds B.Sc (Hons), M.Sc (Hons), and Ph.D. degrees in Horticulture. Listed among H-index–based scientists (2022–23), he has 60+ research articles, and 8+ book chapters. He has participated in 20+ scientific events. His expertise includes pomology, postharvest science, stress physiology, plant genomics, and horticultural crop production.Mona FA Dawood is an Associate Professor at the Botany and Microbiology Department, Assiut University, Egypt. She earned her M.Sc and Ph.D. in abiotic stress and has published 50+ research articles, 5 reviews, 9 book chapters, and 10 conference abstracts. With an H-index of 21, she serves as editor and guest editor for reputed journals and has reviewed over 210 articles. In 2023, she was recognized among Stanford University’s top 2% scientists worldwide.

• Preface. Principles and Practices of CRISPR-Cas9 Genome Editing Techniques. Molecular Mechanisms and Current Progress of CRISPR/Cas in Plant Development. Genome Mapping and Gene Sequencing: Applications of CRISPR/Cas9 in Cereal Crops. CRISPR/Cas Design, DNA Repair Pathways, and Mutation Analysis. CRISPR/Cas9 as an Effective Tool Used for the Improvement of Fruit Crops. Ornamental Crops Improvement Using CRISPR/Cas9. Manipulating the CRISPR/Cas Genome Editing Technology for Targeted Genome Mutagenesis in Ornamental Plants. CRISPR/Cas9 Technology in Engineering Viral and Bacterial Immunity in Agricultural Crops. Defence Mechanisms Developed by CRISPR/Cas in Plants Against Abiotic and Biotic Stresses. Trends in CRISPR/Cas9 Editing Technology as an Approach for Climate-Smart Agriculture. CRISPR/Cas9: An Innovative Biotechnological Approach for the Improvement of Oilseeds. Improvement of Vegetable Crops Using CRISPR-Cas9 as A Gene Editing Tool. CRISPR-Cas Based Genome Editing Strategies Towards Adaptation of Rice Crop to Disease Resistance. Advancement in CRISPR/Cas9 Genome Editing in Field of Crop Improvement. Linking miRNAs and Nano-technology with CRISPR/Cas System for Improvement of Agricultural Crops. Legal, Ethical, and Social Issues Remained in CRISPR/Cas Genome Editing Technology. Index. About the Editors.

Abdul Basit is a Ph.D. researcher at the Department of Horticultural Science, Kyungpook National University, South Korea. He previously worked at the National Agricultural Research Center, Islamabad, and the Climate Change Center, The University of Agriculture, Peshawar. Supported by Pakistani and Korean government scholarships, he has published 67+ articles and 17+ book chapters. He has participated in over 35 scientific events. His research focuses on molecular cytogenetics, plant genomics, breeding, stress physiology, and horticultural crop production.Heba I. Mohamed is a Professor of Plant Physiology at Ain Shams University. Her research focuses on plant stress physiology, biochemistry, eco-friendly stress alleviation, and genetic diversity. She has edited nine books published by international publishers and has also published 40 book chapters, eight reviews, and over 140 research articles. She obtained Encouragement State Award in Basic Sciences (Biological Sciences) 2018 and has been listed among Stanford–Elsevier’s top 2% most influential scientists (2021–2024).Syed Tanveer Shah is an Assistant Professor at the Department of Agriculture, Hazara University, and previously served over a decade in Horticulture at The University of Agriculture, Peshawar. He holds B.Sc (Hons), M.Sc (Hons), and Ph.D. degrees in Horticulture. Listed among H-index–based scientists (2022–23), he has 60+ research articles, and 8+ book chapters. He has participated in 20+ scientific events. His expertise includes pomology, postharvest science, stress physiology, plant genomics, and horticultural crop production.Mona FA Dawood is an Associate Professor at the Botany and Microbiology Department, Assiut University, Egypt. She earned her M.Sc and Ph.D. in abiotic stress and has published 50+ research articles, 5 reviews, 9 book chapters, and 10 conference abstracts. With an H-index of 21, she serves as editor and guest editor for reputed journals and has reviewed over 210 articles. In 2023, she was recognized among Stanford University’s top 2% scientists worldwide.

• Preface. Principles and Practices of CRISPR-Cas9 Genome Editing Techniques. Molecular Mechanisms and Current Progress of CRISPR/Cas in Plant Development. Genome Mapping and Gene Sequencing: Applications of CRISPR/Cas9 in Cereal Crops. CRISPR/Cas Design, DNA Repair Pathways, and Mutation Analysis. CRISPR/Cas9 as an Effective Tool Used for the Improvement of Fruit Crops. Ornamental Crops Improvement Using CRISPR/Cas9. Manipulating the CRISPR/Cas Genome Editing Technology for Targeted Genome Mutagenesis in Ornamental Plants. CRISPR/Cas9 Technology in Engineering Viral and Bacterial Immunity in Agricultural Crops. Defence Mechanisms Developed by CRISPR/Cas in Plants Against Abiotic and Biotic Stresses. Trends in CRISPR/Cas9 Editing Technology as an Approach for Climate-Smart Agriculture. CRISPR/Cas9: An Innovative Biotechnological Approach for the Improvement of Oilseeds. Improvement of Vegetable Crops Using CRISPR-Cas9 as A Gene Editing Tool. CRISPR-Cas Based Genome Editing Strategies Towards Adaptation of Rice Crop to Disease Resistance. Advancement in CRISPR/Cas9 Genome Editing in Field of Crop Improvement. Linking miRNAs and Nano-technology with CRISPR/Cas System for Improvement of Agricultural Crops. Legal, Ethical, and Social Issues Remained in CRISPR/Cas Genome Editing Technology. Index. About the Editors.

Abdul Basit is a Ph.D. researcher at the Department of Horticultural Science, Kyungpook National University, South Korea. He previously worked at the National Agricultural Research Center, Islamabad, and the Climate Change Center, The University of Agriculture, Peshawar. Supported by Pakistani and Korean government scholarships, he has published 67+ articles and 17+ book chapters. He has participated in over 35 scientific events. His research focuses on molecular cytogenetics, plant genomics, breeding, stress physiology, and horticultural crop production.Heba I. Mohamed is a Professor of Plant Physiology at Ain Shams University. Her research focuses on plant stress physiology, biochemistry, eco-friendly stress alleviation, and genetic diversity. She has edited nine books published by international publishers and has also published 40 book chapters, eight reviews, and over 140 research articles. She obtained Encouragement State Award in Basic Sciences (Biological Sciences) 2018 and has been listed among Stanford–Elsevier’s top 2% most influential scientists (2021–2024).Syed Tanveer Shah is an Assistant Professor at the Department of Agriculture, Hazara University, and previously served over a decade in Horticulture at The University of Agriculture, Peshawar. He holds B.Sc (Hons), M.Sc (Hons), and Ph.D. degrees in Horticulture. Listed among H-index–based scientists (2022–23), he has 60+ research articles, and 8+ book chapters. He has participated in 20+ scientific events. His expertise includes pomology, postharvest science, stress physiology, plant genomics, and horticultural crop production.Mona FA Dawood is an Associate Professor at the Botany and Microbiology Department, Assiut University, Egypt. She earned her M.Sc and Ph.D. in abiotic stress and has published 50+ research articles, 5 reviews, 9 book chapters, and 10 conference abstracts. With an H-index of 21, she serves as editor and guest editor for reputed journals and has reviewed over 210 articles. In 2023, she was recognized among Stanford University’s top 2% scientists worldwide.

• Preface. Principles and Practices of CRISPR-Cas9 Genome Editing Techniques. Molecular Mechanisms and Current Progress of CRISPR/Cas in Plant Development. Genome Mapping and Gene Sequencing: Applications of CRISPR/Cas9 in Cereal Crops. CRISPR/Cas Design, DNA Repair Pathways, and Mutation Analysis. CRISPR/Cas9 as an Effective Tool Used for the Improvement of Fruit Crops. Ornamental Crops Improvement Using CRISPR/Cas9. Manipulating the CRISPR/Cas Genome Editing Technology for Targeted Genome Mutagenesis in Ornamental Plants. CRISPR/Cas9 Technology in Engineering Viral and Bacterial Immunity in Agricultural Crops. Defence Mechanisms Developed by CRISPR/Cas in Plants Against Abiotic and Biotic Stresses. Trends in CRISPR/Cas9 Editing Technology as an Approach for Climate-Smart Agriculture. CRISPR/Cas9: An Innovative Biotechnological Approach for the Improvement of Oilseeds. Improvement of Vegetable Crops Using CRISPR-Cas9 as A Gene Editing Tool. CRISPR-Cas Based Genome Editing Strategies Towards Adaptation of Rice Crop to Disease Resistance. Advancement in CRISPR/Cas9 Genome Editing in Field of Crop Improvement. Linking miRNAs and Nano-technology with CRISPR/Cas System for Improvement of Agricultural Crops. Legal, Ethical, and Social Issues Remained in CRISPR/Cas Genome Editing Technology. Index. About the Editors.