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Biomedicine faces critical challenges, including the development of targeted drug delivery systems, the creation of effective antibacterial agents, and the advancement of cancer therapies, among others. This book demonstrates how 2D nanomaterials are emerging as powerful solutions and explores the synthesis, characterization, and functionalization of these materials, unlocking their potential to overcome existing limitations. From gene delivery and tissue regeneration to innovative phototherapy and hyperthermia treatments, the book encompasses a broad range of applications. It explores how 2D nanomaterials are revolutionizing biosensors and bioimaging, enabling earlier and more accurate disease detection. Providing key insights for researchers and students in nanomedicine, materials science, chemistry, and bioengineering, this book offers a roadmap for utilizing 2D nanomaterials to tackle pressing biomedical needs and improve patient outcomes. It focuses on major treatment and diagnostic approaches to solve problems.