RNA Abundance Analysis

Methods and Protocols

AvHailing Jin,Isgouhi Kaloshian

Häftad, Engelska, 2021

Del i serien Methods in Molecular Biology

1 167 kr

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Beskrivning

It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms.

Produktinformation

Utgivningsdatum:2021-08-16
Mått:178 x 254 x 14 mm
Vikt:474 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:233
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071607459

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Naturvetenskap:allmänt inom Naturvetenskap och teknik

Innehållsförteckning

CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana.- Strand-Specific RNA-Seq Applied to Malaria Samples.- Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning.- Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting.- Quantitative Analysis of Plant miRNA Primary Transcripts.- A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq.- Analysis of RBP Regulation and Co-Regulation of mRNA 3’ UTR Regions in a Luciferase Reporter System.- Extraction of Small RNAs by Titanium Dioxide Nanofibers.- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.- Purification and Analysis of Chloroplast RNAs in Arabidopsis.- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes.- Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs.- Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses.- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue.- Studying RNA-Protein Interaction Using Riboproteomics.- Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.