Vaccine Delivery Technology

Methods and Protocols

AvBlaine A. Pfeifer,Andrew Hill

Inbunden, Engelska, 2020

Del i serien Methods in Molecular Biology

2 223 kr

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Beskrivning

This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes.

Produktinformation

Utgivningsdatum:2020-09-22
Mått:178 x 254 x 38 mm
Vikt:1 324 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:590
Förlag:Springer-Verlag New York Inc.
ISBN:9781071607947

Utforska kategorier

Onkologi inom Medicin
Biokemisk teknik inom Naturvetenskap och teknik
Kemi inom Naturvetenskap och teknik

Innehållsförteckning

Vaccine Delivery and Immune Response Basics.- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.- Antigen Discovery in Bacterial Pan-Proteomes.- Alphavirus-Based Antigen Preparation.- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.- Production of Zika Virus Virus-Like Particles.- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.- Whole-Cell Vaccine Preparation: Options and Perspectives.- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.- Attenuation Methods for Live Vaccines.- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.- Lipofection-Based Delivery of DNA Vaccines.- Flavivirus DNA Vaccine Design and Adjuvant Selection.- Vaccine Delivery with a Detoxified Bacterial Toxin.- Needle-Less or Non-Invasive Delivery Technology.- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.- A Hybrid Biological-Biomaterial Vector for Antigen Delivery.- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot.- The Measurement of Antigen-Specific IgG Titers by Direct ELISA.- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.