Neural Reprogramming

Methods and Protocols

AvHenrik Ahlenius

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

2 181 kr

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Beskrivning

This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies.

Produktinformation

Utgivningsdatum:2021-07-30
Mått:178 x 254 x 21 mm
Vikt:729 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:263
Förlag:Springer-Verlag New York Inc.
ISBN:9781071616000

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Biomedicinsk teknik inom Medicin

Innehållsförteckning

Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts.- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons.- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9.- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules.- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons.- Direct Conversion of Human Fibroblasts to Induced Neurons.- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts.- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs).- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors.- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons.- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression.- Transcriptional Profiling During Neural Conversion.- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo.- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods.- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions.- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders.- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.