RNAi Strategies for Pest Management

Methods and Protocols

AvLuis María Vaschetto

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

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Beskrivning

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems.

Produktinformation

Utgivningsdatum:2021-09-09
Mått:178 x 254 x 27 mm
Vikt:918 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:369
Förlag:Springer-Verlag New York Inc.
ISBN:9781071616321

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Innehållsförteckning

Genome-Wide Identification and Functional Characterization of Non-Coding RNAs (ncRNAs) Differentially Expressed during Insect Development.- Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes.- Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi.- Guiding RNAi Design through Characterization of Endogenous Small RNA Pathways.- Silencing of Molecular Targets with Relevance to Insecticide Resistance in Colorado Potato Beetle using dsRNA.- Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization.- In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control.- RNAi Feeding Bioassay: A Protocol for dsRNA Screening against Asian Citrus Psyllid and Related Hemipteran Insects.- A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte).- Rapid Screening of Myzus persicae (Green Peach Aphid) RNAi Targets using Tobacco rattle virus.- Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae.- Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane mosaic virus.- RT-qPCR for Detection and Expression Monitoring of Core Genes Involved in the RNA Interference Pathways of Western Corn Rootworm (Diabrotica virgifera virgifera).- Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control.- Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae.- Plant-Mediated RNA Interference Expressing dsRNA in Cytoplasm for RNAi-Based Pest Control.- RNAi Expression in Cotton for Control of Herbivorous Insects.- Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.- Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115 (DE3) for Non-Transgenic RNAi-Based Insect Pest-Management.- Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.- Application of Nanoparticle-Mediated RNAi for Efficient Gene Silencing and Pest Control on Soybean Aphids.- Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran.- The CRISPR/Cas9-Mediated Genome Editing System in Insect Genomics and Pest Management.