Protoplast Technology

Methods and Protocols

AvKan Wang,Feng Zhang

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

2 100 kr

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Beskrivning

The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species.

Produktinformation

Utgivningsdatum:2022-03-09
Mått:178 x 254 x 21 mm
Vikt:722 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:263
Förlag:Springer-Verlag New York Inc.
ISBN:9781071621639

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Physcomitrium patens Protoplasting and Protoplast Transfection.- Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici.- Protoplast for Gene Functional Analysis in Arabidopsis.- Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum.- CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration.- Protoplast Isolation and Transfection in Rice.- Protoplast Isolation and Transfection in Maize.- Isolation and Transfection of Maize Endosperm Protoplasts.- Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue.- Protoplast Isolation and Transfection in Wheat.- Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses.- Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa).- Protoplast Isolation,Transfection, and Gene Editing for Soybean (Glycine max).- Protoplast Isolation and Transformation in Oil Palm.- Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells.- Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Protoplast Transfection.- High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts.