Transgenesis

Methods and Protocols

Häftad, Engelska, 2023

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This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research.

Transgenesis and Genome Engineering: A Historical Review.- Practical Application of the 3Rs in Rodent Transgenesis.- Genetic and Molecular Quality Control of Genetically Engineered Mice.- Genotyping Genome-Edited Founders and Subsequent Generation.- ASIS-Seq: Transgene Insertion Site Mapping by Nanopore Adaptive Sampling.- High Throughput Analysis of CRISPR-Cas9 Editing Outcomes in Cell and Animal Models Using CRIS.py.- Advanced Technologies and Automation in mES Cell Workflow.- Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System.- Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs.- CRISPR/Cas9 Endonuclease Mediated Mouse Genome Editing of 1 Cell and/or 2 Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Inter Homolog Repair Mechanism.- Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes.- CRISMERE Chromosome Engineering in Mouse and Rat.- Targeted Integration of Transgenesat the Mouse Gt(ROSA)26Sor Locus.- Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes.- Gene Targeting in Rat Embryonic Stem Cells.- Rat Embryonic Stem Cell Transgenesis.- CRISPR/Cas9-Mediated Genome Editing in Zebrafish.- Generation of Rabbit Chimeras by 8-Cell Stage Embryo Injection.- Genome Editing in Pigs.- Generation of Genome-Edited Chicken through Targeting of Primordial Germ Cells.