Hepatic Stellate Cells

Methods and Protocols

AvRalf Weiskirchen,Scott L. Friedman

Häftad, Engelska, 2023

Del i serien Methods in Molecular Biology

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Beskrivning

This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism.

Produktinformation

Utgivningsdatum:2023-05-29
Mått:178 x 254 x 18 mm
Vikt:612 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:309
Förlag:Springer-Verlag New York Inc.
ISBN:9781071632093

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update.- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells.- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells.- Phalloidin Staining for F-Actin in Hepatic Stellate Cells.- Retinyl Ester Analysis by Orbitrap Mass Spectrometry.- Studying Hepatic Stellate Cell Senescence.- Isolation of Hepatic Stellate Cells and Lymphocytes for Co-Culture Systems.- Working with Immortalized Hepatic Stellate Cell Lines.- Induction of Obstructive Cholestasis in Mice.- Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation.- Generation and Culture of Primary Mouse Hepatocyte-HSC Spheroids.- Hepatic Stellate Cell Depletion and Genetic Manipulation.- Human Hepatic Stellate Cells: Isolation and Characterization.- Decellularization of Human Liver to Generate Native Extracellular Matrix for Use in Automated Functional Assays with Stellate Cells.- Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers.- Single Cell Secretome Analyses of Hepatic Stellate Cells: Aiming for Single Cell Phenomics.- Hepatic Stellate Cells Targeting Using Peptide-Modified Biologicals.- Experimental Workflow for Preclinical Studies of Human Antifibrotic Therapies.