Fluorescence In Situ Hybridization (FISH)

Methods and Protocols

AvGal Haimovich

Inbunden, Engelska, 2024

Del 2784 i serien Methods in Molecular Biology

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Beskrivning

This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments.

Produktinformation

Utgivningsdatum:2024-03-20
Mått:178 x 254 x 23 mm
Vikt:794 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:302
Upplaga:2024
Förlag:Springer-Verlag New York Inc.
ISBN:9781071637654

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in the Fungal Pathogen Candida albicans.- RNA and Protein Detection by Single-Molecule Fluorescent In Situ Hybridization (smFISH) Combined with Immunofluorescence in the Budding Yeast S. cerevisiae.- Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis.- SABER-FISH in Hydractinia.- smFISH for Plants.- Fluorescent In Situ Detection of Small RNAs in Plants using sRNA-FISH.- hcHCR: High-Throughput Single-Cell Imaging of RNA in Human Primary Immune Cells.- High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants.- Simultaneous In Situ Detection of m6A-Modified and Unmodified RNAs using DART-FISH.- Multiplexed Immunofluorescence and Single Molecule RNA Fluorescence InSitu Hybridization in Mouse Skeletal Myofibers.- Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP.- Efficient Prediction Model of mRNA End-to-End Distance and Conformation: Three-Dimensional RNA Illustration Program (TRIP).- Combined 3D DNA FISH, Single Molecule RNA FISH, and Immunofluorescence.- Determining the Compaction State of Genes using DNA FISH.- Hi-M: A Multiplex Oligopaint FISH Method to Capture Chromatin Conformations In Situ and Accompanying Open-Source Acquisition Software.- Rapid DNA-FISH in Arabidopsis thaliana Somatic Cells.- DBD-FISH Using Specific Chromosomal Region Probes for the Study of Cervical Carcinoma Progression.- CRISPR-Based Split Luciferase as a Biosensor for Unique DNA Sequences In Situ.