Zymography

Principles, Process, and Pitfalls, Volume 1

AvRaouf A. Khalil

Inbunden, Engelska, 2025

Del i serien Methods in Molecular Biology

2 143 kr

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Beskrivning

Part II provides details on the use of zymography to assess various endopeptidases, and includes updates on protease zymography, protocols for gelatin zymography, detection of gelatinases by substrate zymography, unveiling matrix metalloproteinase (MMP) dynamics, and measuring MMPs activity by gelatin zymography.

Produktinformation

Utgivningsdatum:2025-05-11
Mått:178 x 254 x 22 mm
Vikt:741 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:270
Förlag:Springer-Verlag New York Inc.
ISBN:9781071644775

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Gelatin Zymography: Principle, Method, and Application in Cancer Research.- Recent Updates on Protease Zymography.- Gelatin Zymography: A Simple and Effective Technique to Detect Gelatinases.- Detection of Gelatinases by Substrate Zymography.- Zymography: Unveiling Matrix Metalloproteinase Dynamics.- Measuring Matrix Metalloproteinases Activity by Gelatin Zymography.- A Practical View of In-Gel Gelatin Zymography of Matrix Metalloproteinases 2 and 9 in Cardiac Tissue.- Gel Zymography for the Evaluation of Matrix Metalloproteinase-2 and -9 in Aorta and Kidney Tissues.- Analysis of Enzymatic Activity of Matrix Metalloproteinases by Collagen Zymography Utilizing Extracted Collagen from Bovine Muscle Tissue.- Electrophoretic Zymography of Protease using Azocasein.- Advanced Zymographic Techniques for Detection and In-Depth Characterization of Microbial Proteases in Enzymology Research.- In-Gel Activity Assay for Glycosidases.- Uncovering Hyaluronidase Activity Using Hyaluronan Zymography in Biological Samples.- Leveraging Zymography to Discover and Investigate Nuclear Proteases.- An Overview of Chitinases Zymography: Past and Present Methods and Protocols.- One- and Two-Dimensional Zymography Methods for Assessing the Family of Proteases.- Loading-Controlled Zymography.- Gel Diffusion-Based Gelatin Zymography to Analyze MMP-2 and MMP-9 Activity in Cell Culture.- Analysis of Protein Glycation Inhibition using Polyacrylamide Gel Electrophoresis.- Analysis of Glycation-Induced Protein Cross-Linking Inhibition using SDS-Polyacrylamide Gel Electrophoresis.- Kinetics of Protease Thermal Inactivation.- Reversible Inhibition of Cysteine Proteases using S-methyl Methanethiosulfonate.