Zymography

Biological and Clinical Applications, Volume 2

AvRaouf A. Khalil

Inbunden, Engelska, 2025

Del 2918 i serien Methods in Molecular Biology

2 325 kr

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Beskrivning

Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates.

Produktinformation

Utgivningsdatum:2025-04-23
Mått:178 x 254 x 22 mm
Vikt:756 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:280
Förlag:Springer-Verlag New York Inc.
ISBN:9781071644812

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

In Situ Zymography and Localization of Bright Green Fluorescent Gelatinase Activity in Tissue Sections.- In Situ Zymography in Formalin-Fixed Paraffin-Embedded and Mineralized Tissues.- In Vivo Zymography: An Essential Activity Assay for Studying the Activity of Matrix Metalloproteinases in Specific Brain Cells.- Fundamentals of Zymography: Applications to the Study of Biological Samples.- Gelatin Zymography to Quantify Levels of MMP-2 and MMP-9 in Complex Biological Samples.- Detection of Proteolytic Enzymes in Polyacrylamide Gel Supplemented with Diverse Biological Substrates.- Evaluating Matrix Metalloproteinase Expression in Primary Mouse Glial Cultures Using Gel Zymography.- Zymography as a Tool for Exploring Protease Activity in Dental Research.- Zymography Techniques for the Profiling of Digestive Protease in a Freshwater Invertebrate Model.- Casein Zymography: A Rapid and Robust Methodology for Detecting Calpains Proteases Activity in Meat Product.- Cost-Effective Method for the Detection of Lipases and Proteases in Crude Extracts of Microbial Proteins Using Gel Zymography.- Applying Zymography Methods for the Detection of Enzymes from Fungal Endophytes.- Assessing MMP-2/9 Proteolytic Activity and Activation Status by Zymography in Preclinical and Clinical Tissue Samples.- Using Zymography to Assess Circulating Matrix Metalloproteinase (MMP)-2 and MMP-9 in Clinical Samples.- Zymography: A Simple and Powerful Tool for the Assessment of MMP-2 and MMP-9 in Pathological Conditions.- Zymography for the Detection of Bacterial Proteases.- Gelatin Zymography of Proteases in Chronic Wound Samples.- In-Gel Zymography of Amyotrophic Lateral Sclerosis-Associated Variants of Superoxide Dismutase-1.- Assessing Gelatinase Activity in Normal and Disease Uterine Tissue and Cells via Gelatin Zymography.- Gelatin Zymography of Conditioned Media from Tumor Cell Lines.- Analysis of Metalloproteinase Secretion in Cancer Cells by Zymography.- Evaluating Matrix Metalloproteinase Activity in Human Hepatocellular Carcinoma Tissues and Cells with Gelatin Zymography.- Gelatin Zymography: Detecting Gelatinase Activity as a Partner for Invadopodia-Mediated Invasion by Tumor Cells.