Brain Tumor Stem Cells

Methods and Protocols

AvSheila K. Singh,Chitra Venugopal

Inbunden, Engelska, 2025

Del 2944 i serien Methods in Molecular Biology

1 868 kr

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Beskrivning

mso-bidi-language: AR-SA;">This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation.

Produktinformation

Utgivningsdatum:2025-06-25
Mått:178 x 254 x 24 mm
Vikt:814 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:311
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071646533

Utforska kategorier

Onkologi inom Medicin
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Brain Tumor Stem Cells: New Perspectives.- Isolation and Propagation of Brain Tumor Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Isolation and Culture of Human Neural Stem Cells.- Metabolic Assays for Brain Cancer Cells.- Synergy Assay for Screening Small Molecule Combinations in Brain Cancer Stem Cells.- Extracellular Flux Assays for the Measurement of Glycolysis and Mitochondrial Respiration in Brain Cancer Cells.- O6-Methylguanine–DNA Methyltransferase (MGMT) Promoter Methylation Analysis in Glioblastoma Patients.- Profiling Glioma Stem Cell Dynamics via 3D-based Cell Cycle Reporter Assays.- Proximity Ligation Assay.- Efficient Migration Assay for Brain Tumor Stem Cells.- Patient-Derived Glioblastoma Explants Empower Rapid and Personalized Drug Assessment: Harnessing the Potential of 3D Perfusion Bioreactors in Glioblastoma Drug Discovery.- Chimeric Antigen Receptor T Cell Production for Brain Cancers.- CRISPR-Cas9-Guided Genetic Manipulation of Patient-Derived Brain Tumor Cells.- Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry.- In Vitro Models to Compare the Duration of Action of New Therapeutic Scaffolds and Drug Eluting Medical Devices.- Cleavage Under Targets & Release Using Nuclease (CUT&RUN) Protocols for the Study of Genetic and Epigenetic Regulation in Cancer.- A Toolkit for Single-Nucleus Characterization of Glioblastoma.- Using GlioVis to Explore, Analyze, and Visualize Brain Tumor Multi-Omics Datasets.- Modeling Glioma Stem Cell-Mediated Tumorigenesis Using Zebrafish Patient-Derived Xenograft Systems.- An In Vivo Model of Recurrent Glioblastoma.- Bioluminescent Imaging of Patient-Derived Brain Tumors in Live Mice.- Growth Dynamics of Brain Tumor through the Lens of MT-Weighted MRI.