Sheila K. Singh – författare

This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation. The book collects up-to-date methodologies to isolate and propagate BTSCs and to study them using various cutting-edge techniques, in service of bringing us closer to translating the discoveries made from these cells into new therapeutic options for brain cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Brain Tumor Stem Cells: Methods and Protocols, Second Edition provides a comprehensive understanding of the skills and techniques needed to unlock data from this most informative subset of cells.

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.