Brain Tumor Stem Cells

Methods and Protocols

AvSheila K. Singh,Chitra Venugopal

Inbunden, Engelska, 2018

Del i serien Methods in Molecular Biology

1 094 kr

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Beskrivning

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse.

Produktinformation

Utgivningsdatum:2018-10-16
Mått:178 x 254 x 20 mm
Vikt:704 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:254
Förlag:Humana Press Inc.
ISBN:9781493988044

Utforska kategorier

Onkologi inom Medicin
Biovetenskap inom Naturvetenskap och teknik
Neurologi och klinisk neurofysiologi inom Medicin

Recensioner i media

“Sheila K. Singh and Chitra Venugopal have developed a concise, systematic, and practical book; an ideal read for brain cancer researchers. … this book is recommended for anyone who strives for proficiency in biophysiology of cancer stem cells and hopes to discover novel therapeutic and treatment strategies for patients affected by devastating CNS disease.” (Angela M. Bohnen, Neurosurgery, Vol. 85 (4), October, 2019)

Innehållsförteckning

Introduction to Brain Tumor Stem Cells.- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells.- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors.- Detecting Stem Cell Marker Expression Using the NanoString nCounter System.- Flow Cytometric Analysis of Brain Tumor Stem Cells.- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells.- Differentiation of Brain Tumor Initiating Cells.- The Study of Brain Tumor Stem Cell Migration.- The Study of Brain Tumor Stem Cell Invasion.- Cell Cycle Dynamics in Glioma Cancer Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.- In Vitro Assays for Screening Small Molecules.- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma.- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci.- In Vivo Murine Models of Brain Metastasis.- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.