Testing and Analysis of GMO-containing Foods and Feed

Salah Eldin Osman Mahgoub is a Certified Food Scientist (CFS), Professional member of the Institute of Food Technologists (IFT,USA), and Fulbright scholar with international work experience. Has been a university professor in the university of Botswana, Botswana, university of Khartoum, Sudan and an adjunct professor at Kaplan University, USA. A critical thinker with proven experience in food science, food processing, genetically modified foods, functional foods and nutrition in relation to various consumer issues. An experienced researcher, team leader and effective team player, with proven track on developing products from the concept to the shelf and the impact on consumer preferences. Published 34 articles and conference proceedings in peer reviewed journals. Presented and participated in 42 conferences and workshops globally.Leo M.L. Nollet, PhD, received an MS (1973) and PhD (1978) in biology from the Katholieke Universiteit Leuven, Belgium. He is an editor and associate editor of numerous books. He edited for M. Dekker, New York—now CRC Press of Taylor & Francis—the first, second, and third editions of the books entilted Food Analysis by HPLC and Handbook of Food Analysis. The last edition is a two volume book. He also edited the Handbook of Water Analysis (first, second, and third editions) and Chromatographic Analysis of the Environment, Third Edition (CRC Press). With F. Toldrá he coedited two books published in 2006 and 2007: Advanced Technologies for Meat Processing (CRC Press) and Advances in Food Diagnostics (Blackwell Publishing—now Wiley). With M.Poschl he coedited the book Radionuclide Concentrations in Foods and the Environment also published in 2006 (CRC Press). Dr. Nollet has also coedited with Y. H. Hui and other colleagues several books: Handbook of Food Product Manufacturing (Wiley,2007), Handbook of Food Science, Technology and Engineering (CRC Press, 2005), Food Biochemistry and Food Processing (first and second editions; Blackwell Publishing—now Wiley—2006 and 2012), and the Handbook of Fruits and Vegetable Flavors(Wiley, 2010). In addition, he edited the Handbook of Meat, Poultry and Seafood Quality, first and second editions, (Blackwell Publishing—now Wiley—2007 and 2012). From 2008 to 2011, he published with F. Toldrá five volumes on animal product–related books.

• ContentsPreface ixList of Contributors xiiiAuthor xvSection 1 GMOsChapter 1 Genetically Modified Organisms 3Salah E. O. MahgoubSection 2 SAMPLINGChapter 2 Guidelines for Sample Preparation Procedures inGMO Analysis 47European CommissionSection 3 GMO TESTING METHODSChapter 3 Immunoassays (Protein-Based Methods) 91Rajesh Kumar, Vishal Srivashtav, and Abhilasha TripathiChapter 4 PCR Techniques for Detection and Quantification of GMOs 115Frédéric Debode and Gilbert BerbenChapter 5 Sensors 155Beatriz López-Ruiz, Marta Sánchez-Paniagua López, andCarmen Lorena Manzanares-PalenzuelaChapter 6 The Application of Nucleic Acid Microarrays for theDetection of Genetically Modified Organisms 205Stuart J. LucasChapter 7 Recent Developments in Detection Methods of GeneticallyModified Organisms 223Leo M.L. NolletSection 4 VALIDATION AND STANDARDIZATION OF METHODSChapter 8 Verification of Analytical Methods for GMO Testing WhenImplementing Interlaboratory Validated Methods 245JRC Technical ReportHougs Lotte, Francesco Gatto, O. Goerlich, Lutz Grohmann,K. Lieske, Marco Mazzara, Frank Narendja, J. Ovesna, NinaPapazova, I.M.J. Scholtens, Jana ZelChapter 9 Reference Materials for GMO Analysis 267Leo M.L. NolletChapter 10 ISO (International Organization for Standardization) 273Leo M.L. NolletChapter 11 Reference Methods for GMO Analysis 277Leo M.L. NolletIndex 283