Protein Engineering and Design

Sheldon Park holds a B.A. in math and physics from the University of California (Berkeley), an M.S. in physics from Massachusetts Institute of Technology, and a Ph.D. in biophysics from Harvard University. He studied protein engineering and design while working as a postdoc for Dr. Jeffery Saven and Dr. Eric Boder at the University of Pennsylvania. Since 2006, he has been a professor of chemical and biological engineering at University at Buffalo. In his research, Dr. Park uses modeling and simulation to analyze protein molecules and uses high-throughput screening to engineer protein molecules of various structure and function. He is particularly interested in developing efficient methods of engineering complex protein molecules with potential biotechnological and biomedical applications. Jennifer Cochran holds a B.S. in biochemistry from the University of Delaware and a Ph.D. in biological chemistry from Massachusetts Institute of Technology (MIT). She studied and developed combinatorial protein engineering methods while a postdoctoral fellow in the lab of K. Dane Wittrup in the Department of Biological Engineering at MIT. Since 2005, she has been a professor of bioengineering at Stanford University. Dr. Cochran's laboratory uses interdisciplinary approaches in chemistry, engineering, and biophysics tostudy complex biological systems and to create designer protein therapeutics and diagnostic agents for biomedical applications. She is interested in elucidating molecular details of receptor-mediated cell signaling events and at the same time developing protein and polymer-based tools that will allow manipulation of cell processes on a molecular level.

• Phage Display Systems for Protein Engineering. Cell Surface Display Systems for Protein Engineering. Cell-Free Display Systems for Protein Engineering. Library Construction for Protein Engineering. Design and Engineering of Synthetic Binding Proteins Using Nonantibody Scaffolds. Combinatorial Enzyme Engineering. Engineering of Therapeutic Proteins. Protein Engineered Biomaterials. Protein Engineering Using Noncanonical Amino Acids. Computer Graphics, Homology Modeling, and Bioinformatics. Knowledge-Based Protein Design. Molecular Force Fields. Rotamer Libraries for Molecular Modeling and Design of Proteins. Search Algorithms. Modulating Protein Structure. Modulation of Intrinsic Properties by Computational Design. Modulating Protein Interactions by Rational and Computational Design. Future Challenges of Computational Protein Design.