Single-Channel Recording

Häftad, Engelska, 2012

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Beskrivning

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals.

Produktinformation

Utgivningsdatum:2012-04-26
Mått:178 x 254 x 29 mm
Vikt:982 g
Format:Häftad
Språk:Engelska
Antal sidor:504
Upplaga:1983
Förlag:Springer-Verlag New York Inc.
ISBN:9781461578604

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

I. Methods.- 1 Electronic Design of the Patch Clamp.- 2 Geometric Parameters of Pipettes and Membrane Patches.- 3 Science and Technology of Patch-Recording Electrodes.- 4 Enzymatic Dispersion of Heart and Other Tissues.- 5 A Primer in Cell Culture for Patchologists.- 6 Patch-Clamped Liposomes: Recording Reconstituted Ion Channels.- 7 Tight-Seal Whole-Cell Recording.- 8 The Loose Patch Clamp.- II. Concepts and Analysis.- 9 The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- 10 Conformational Transitions of Ionic Channels.- 11 Fitting and Statistical Analysis of Single-Channel Records.- 12 Automated Analysis of Single-Channel Records.- 13 Analysis of Nonstationary Channel Kinetics.- 14 An Example of Analysis.- 15 Membrane Current and Membrane Potential from Single-Channel Kinetics.- III. Patch Clamp Data.- 16 Bursts of Openings in Transmitter-Activated Ion Channels.- 17 Is the Acetylcholine Receptor a Unit-Conductance Channel?.- 18 Analysis of Single-Channel Data from Glutamate Receptor-Channel Complexes on Locust Muscle.- 19 Experimental Approaches Used to Examine Single Glutamate-Receptor Ion Channels in Locust Muscle Fibers.- 20 Cholinergic Chloride Channels in Snail Neurons.- 21 Single-Channel Analysis in Aplysia Neurons: A Specific K + Channel is Modulated by Serotonin and Cyclic AMP.- 22 Cholecystokinin and Acetylcholine Activation of Single-Channel Currents via Second Messenger in Pancreatic Acinar Cells.- 23 Observations on Single Calcium Channels: An Overview.- 24 Potassium and Chloride Channels in Red Blood Cells.- 25 The Influence of Membrane Isolation on Single Acetylcholine-Channel Current in Rat Myotubes.