Fish Cytogenetic Techniques

Catherine Ozouf-Costaz, Eva Pisano, Fausto Foresti, Lurdes Foresti de Almeida Toledo

• Teleost Fish Handling and Transport under Reduced Stress Conditions. Storage of Karyotyped Voucher Specimens and their Molecular Identification. Direct Chromosome Preparation from Freshwater Teleost Fishes. Direct Mitotic Chromosome Preparations from Chondrichthyan Tissues. Mitotic Chromosome Preparations of Freshwater Stingrays. Direct Chromosome Preparation from Regenerating Fish Fin Tissue. Direct Chromosome Preparations from Embryos and Larvae. Establishment of Sturgeon Primary Cell Lines. Teleost Fish Lymphocyte Culture. Rapid Fibroblast Culture for Teleost Fish Karyotyping. Cephalic Kidney and Spleen Cell Culture in Antarctic Teleosts. Meiotic Surface-spread and Immunodetection for Synaptonemal Complex Proteins in Teleost fish. C-banding. Sequential Chromosome Banding in Fishes. Standard Fluorescence in Situ Hybridization Procedures. Characterization of Fish Genomes by GISH and CGH. Fish Chromosome Microdissection and its Utilization in the Synthesis of Fluorescent in situ Hybridization Probes. Microdissection and Chromosome Painting. Extending DNA Fibers and Fiber-FISH. In vivo Micronucleus Test for Detecting Response of Teleost Fish to Environmental Genotoxic Exposure.