Fish Cytogenetic Techniques

Ray-Fin Fishes and Chondrichthyans

AvCatherine Ozouf-Costaz,Eva Pisano

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PDF, Engelska, 2015

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Beskrivning

Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chro

Produktinformation

Utgivningsdatum:2015-06-17
Språk:Engelska
Filformat:PDF
Kopieringsskydd:LCP
ISBN:9781482211993
Förlag:Taylor & Francis Ltd

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Catherine Ozouf-Costaz, Eva Pisano, Fausto Foresti, Lurdes Foresti de Almeida Toledo

Innehållsförteckning

Teleost Fish Handling and Transport under Reduced Stress Conditions. Storage of Karyotyped Voucher Specimens and their Molecular Identification. Direct Chromosome Preparation from Freshwater Teleost Fishes. Direct Mitotic Chromosome Preparations from Chondrichthyan Tissues. Mitotic Chromosome Preparations of Freshwater Stingrays. Direct Chromosome Preparation from Regenerating Fish Fin Tissue. Direct Chromosome Preparations from Embryos and Larvae. Establishment of Sturgeon Primary Cell Lines. Teleost Fish Lymphocyte Culture. Rapid Fibroblast Culture for Teleost Fish Karyotyping. Cephalic Kidney and Spleen Cell Culture in Antarctic Teleosts. Meiotic Surface-spread and Immunodetection for Synaptonemal Complex Proteins in Teleost fish. C-banding. Sequential Chromosome Banding in Fishes. Standard Fluorescence in Situ Hybridization Procedures. Characterization of Fish Genomes by GISH and CGH. Fish Chromosome Microdissection and its Utilization in the Synthesis of Fluorescent in situ Hybridization Probes. Microdissection and Chromosome Painting. Extending DNA Fibers and Fiber-FISH. In vivo Micronucleus Test for Detecting Response of Teleost Fish to Environmental Genotoxic Exposure.