Peptide Libraries

Methods and Protocols

AvRatmir Derda

Inbunden, Engelska, 2015

Del 1248 i serien Methods in Molecular Biology

1 092 kr

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Beskrivning

This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Produktinformation

Utgivningsdatum:2015-01-24
Mått:178 x 254 x 22 mm
Vikt:758 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:278
Upplaga:2015
Förlag:Humana Press Inc.
ISBN:9781493920198

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries.- Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization.- Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries.- Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation.- Macrocyclic Templates for Library Synthesis of Peptido-Conjugates.- Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2α Receptor Modulators as Therapeutics to Inhibit Preterm Labour.- A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases.- Peptide and Peptide Library Cyclization Via Bromomethyl Benzene Derivatives.- Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges.- De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry.- Chemical Post-Translational Modification of Phage-Displayed Peptides.- Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-Scanning.- Identifying Reactive Peptides from Phage-Displayed Libraries.- The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags.- Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries.- Screening Peptide Array Library for the Identification of Cancer Cell Binding Peptides.- Next Generation Sequencing of Phage-Displayed Peptide Libraries.- Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries.