Histology Protocols

From the reviews:“This slim volume aims to provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques to realise their potential of their resource. … I enjoyed reading this book … . this book is not particularly expensive and is so comprehensive and well written that I would certainly recommend it to anyone thinking of setting up a research project but uncertain what sort of method to use. It would also be a valuable asset to any departmental library … .” (Anne Patricia Campbell, Bulletin of the Royal Society of Pathologists, Issue 154, April, 2011)“Reports the basic techniques of immunohistochemistry and molecular biology used to optimize resources and obtain accurate results. … This book is recommended to students and researchers using the laboratory techniques on histology described. Updated protocols are described by renowned scientists in the area using understandable language while details of possible interference are given for each technique. Tim D. Hewiston and Ian A. Darby have undertaken several studies on histology, many of which were conducted in collaboration with the authors contributing to this book.” (Rui Curi, Brazilian Journal of Pharmaceutical Sciences, Spring, 2011)“This volume in the Methods in Molecular Biology series provides a compendium of proven protocols for modern histology. … Written for basic science and clinical researchers interested in mapping the location and distribution of biomarkers, the book also will be appreciated by lecturers and students specializing in biomedical technology, histology, and clinical laboratory medicine. … The book is well written and carefully edited, and will be a welcome resource for all basic science and clinical researchers studying modern cell biology.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

• Tissue Processing.- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy.- An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue.- Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus.- Staining Techniques.- Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells.- Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems.- Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections.- High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy.- Lectin Histochemistry for Light and Electron Microscopy.- Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain.- Special Stains for Extracellular Matrix.- Active Staining of Mouse Embryos for Magnetic Resonance Microscopy.- Immunohistochemical Detection of Tumour Hypoxia.- In Situ Localization of Apoptosis Using TUNEL.- Imaging Techniques.- Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord.- High-Resolution Confocal Imaging in Tissue.- Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography.- Image Analysis and Quantitative Morphology.