Cell-Free Protein Production

Methods and Protocols

AvYaeta Endo,Kazuyuki Takai

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

1 316 kr

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Beskrivning

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 16 mm
Vikt:525 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:261
Förlag:Humana Press Inc.
ISBN:9781493956982

Utforska kategorier

Innehållsförteckning

Cell-Free Protein Preparation Through Prokaryotic Transcription–Translation Methods.- PURE Technology.- The Cell-Free Protein Synthesis System from Wheat Germ.- A Cell-Free Protein Synthesis System from Insect Cells.- Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells.- Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System.- Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate.- An Efficient Approach to the Production of Vaccines Against the Malaria Parasite.- Protein Engineering Accelerated by Cell-Free Technology.- Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds.- NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System.- Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination.- Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography.- Production of Multi-Subunit Complexes on Liposome Through an E. coli Cell-Free Expression System.- Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System.- Strategies for the Cell-Free Expression of Membrane Proteins.- Production of Membrane Proteins Through the Wheat-Germ Cell-Free Technology.- Ribosome Display with the PURE Technology.- Incorporation of 3-Azidotyrosine into Proteins Through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification.- Synthesis of Functional Proteins Within Liposomes.