Genomic Imprinting

Methods and Protocols

AvNora Engel

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

1 534 kr

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Beskrivning

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 17 mm
Vikt:590 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:297
Förlag:Humana Press Inc.
ISBN:9781493959662

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Uniparental Embryos in the Study of Genomic Imprinting.- Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species.- Generation of Trophoblast Stem Cells.- Immunomagnetic Purification of Murine Primordial Germ Cells.- Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA.- Identification of Imprinted Loci by Transcriptome Sequencing.- Data Mining as a Discovery Tool for Imprinted Genes.- Engineering of Large Deletions and Duplications In Vivo.- Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells.- Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains.- Quantitative Chromosome Conformation Capture.- Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing.- Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies.- Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs.- Co-Immunoprecipitation of Long Non-Coding RNAs.- Specialized Technologies for Epigenetics in Plants.- Computational Studies of Imprinted Genes.- Insights on Imprinting from Beyond Mice and Men.- Non-Mammalian Parent-Of-Origin Effects.