NMDA Receptors

Methods and Protocols

AvNail Burnashev,Pierre Szepetowski

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

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Beskrivning

This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing.

Produktinformation

Utgivningsdatum:2018-08-14
Mått:178 x 254 x 18 mm
Vikt:619 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:314
Förlag:Humana Press Inc.
ISBN:9781493984527

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

NMDA Receptors in the Central Nervous System.- Quantification of NMDAR Subunit Genes Expression by qRT-PCR.- Genetic and Functional Analysis of GRIN2A in Tumor Samples.- Detection of NMDARs Antibodies in Encephalitis.- MagnetofectionTM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells.- Transfection in Primary Cultured Neuronal Cells.- Selective Cell-Surface Expression of Triheteromeric NMDA Receptors.- Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application System.- GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging.- Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers.- Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations.- Electrophysiological Investigation of NMDA Current Properties in Brain Slices.- Analysis of Functional NMDA Receptors in Astrocytes.- GluNs Detection and Functions in Microglial Cells.- NMDA ReceptorActivity in Circulating Red Blood Cells: Methods of Detection.- NMDA Receptors as Voltage Sensors.- Development of a Computational Approach/Model to Explore NMDA Receptors Functions.