Chimeric RNA

Methods and Protocols

AvHui Li,Justin Elfman

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

1 204 kr

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Beskrivning

This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes.

Produktinformation

Utgivningsdatum:2020-11-14
Mått:178 x 254 x 16 mm
Vikt:525 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:262
Förlag:Humana Press Inc.
ISBN:9781493999064

Utforska kategorier

Onkologi inom Medicin
Biovetenskap inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Prediction, Characterization, and In Silico Validation of Chimeric RNAs.- Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope.- Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras.- Gene Fusion Discovery with INTEGRATE.- Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit.- Detection and Measurement of Chimeric RNAs by RT-PCR.- Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells.- RNase Protection Assay.- Validation of Chimeric Fusion Peptides Using Proteomics Data.- NanoString nCounter Technology: High-Throughput RNA Validation.- Knockdown of Chimeric RNA by RNAi.- Overexpression of Chimeric RNA by Retroviral Transduction.- Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization.- Confirmation of Transcriptional Read-Through Events byRT-PCR.- Validating Gene Fusion as the Source of Chimeric RNAs.- Chimeric RNA and Exosomes-Based Liquid Biopsy.- SMaRT for Therapeutic Purposes.- Case Study: Landscape of Chimeric RNAs in Bladder Cancer.- Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.