Epigenetics Protocols

AvTrygve O. Tollefsbol,Trygve O. Tollefsbol

Inbunden, Engelska, 2004

Del i serien Methods in Molecular Biology

1 122 kr

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Beskrivning

A collection of state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer.

Produktinformation

Utgivningsdatum:2004-07-23
Mått:155 x 235 x 25 mm
Vikt:705 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:304
Upplaga:2004
Förlag:Humana Press Inc.
ISBN:9781588293367

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Recensioner i media

"Comprehensive and easy to use...offers investigators readily reproducible techniques that will further promote progress in this critically important field." - Tumori

Innehållsförteckning

Methods of Epigenetic Analysis.- Chromatin Immunoprecipitation Assays.- Native Chromatin Immunoprecipitation.- Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation.- Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure.- Measuring Changes in Chromatin Using Micrococcal Nuclease.- DNaseI Hypersensitivity Analysis of Chromatin Structure.- Inhibition of Histone Deacetylases.- Site-Specific Analysis of Histone Methylation and Acetylation.- Analysis of Mammalian Telomere Position Effect.- Activity Assays for Poly-ADP Ribose Polymerase.- Multigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns.- Profiling DNA Methylation by Bisulfite Genomic Sequencing.- Methylation-Sensitive Single-Strand Conformation Analysis.- SIRPH Analysis.- Real-Time PCR-Based Assay for Quantitative Determination of Methylation Status.- Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA.- Photocrosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation Analysis.- Methylation-Specific Oligonucleotide Microarray.- Methylation-Specific PCR In Situ Hybridization.- Relative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR Assay.- DMB (DNMT-Magnetic Beads) Assay.