Ancient DNA

Methods and Protocols

AvBeth Shapiro,Michael Hofreiter

Inbunden, Engelska, 2012

Del i serien Methods in Molecular Biology

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Beskrivning

Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra.

Produktinformation

Utgivningsdatum:2012-01-16
Mått:178 x 254 x 19 mm
Vikt:644 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:247
Upplaga:2012
Förlag:Humana Press Inc.
ISBN:9781617795152

Utforska kategorier

Biologi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Setting Up An Ancient DNA Laboratory.-A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples.-DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles.-Case Study: Recovery of ancient nuclear DNA from Toe Pads of the Extinct Passenger Pigeon.-Extraction of DNA from Paleofeces.-DNA Extraction from Keratin and Chitin.-Case Study: Ancient sloth DNA Recovered from Hairs Preserved in Paleofeces.-Ancient DNA Extraction from Soils and Sediments.-DNA Extraction from Fossil Eggshell.-Ancient DNA Extraction from Plants.-DNA Extraction from Formalin-fixed Material.-Case Study: Ancient DNA recovered from Pleistocene-age Remains of a Florida Armadillo.-Non-destructive DNA Extraction from Museum Specimens.-Case Study: Using a Non-destructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens .-PCR Amplification, Cloning and Sequencing of Ancient DNA.-Quantitative Real-time PCR in aDNA Research.-Multiplex PCR Amplification of Ancient DNA.-Preparation of Next-generation Sequencing Libraries from Damaged DNA.-Generating Barcoded Libraries for Multiplex High-throughput Sequencing.-Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS).-Target Enrichment via DNA Hybridization Capture.-Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture.-Analysis of High-throughput Ancient DNA Sequencing Data.-Phylogenetic Analysis of Ancient DNA using BEAST.