UHPLC in Life Sciences

Davy Guillarme gained his PhD in analytical chemistry from the University of Lyon (France) in 2004. He is now lecturer at the School of Pharmaceutical Sciences, University of Geneva (Switzerland). He is working mainly on the development of new approaches to perform ultra-fast and high resolution separations in liquid chromatography. He is also interested in the coupling of these strategies with alternative detection modes, particularly mass spectrometry. Jean-Luc Veuthey obtained his PhD in analytical chemistry from the University of Geneva (Switzerland) in 1987. He is now full professor at the School of Pharmaceutical Sciences, University of Geneva (Switzerland). His interests include the development of LC and CE hyphenated to several detection modes for the analysis of drugs and metabolites. Sample preparation and validation of the procedures are also particularly studied in his laboratory.

The editors of this book have made a great effort to cover all aspects of UHPLC and its application in life sciencesThis book, Volume 16 in the well-known series of RSC chromatography monographs, also has a clear layout, good quality of figures and can be recommended to both students of separation science and professionals using UHPLC.

• 1.1. Trends in HPLC;1.2. Comparison of Chromatographic Techniques and Supports;1.3. Constructing Kinetic Plots;1.4. History of the Kinetic Plot Method;1.5 Unification of the Isocratic and Gradient Kinetic Plot Method;1.6. Relation between the Kinetic Performance under Isocratic and Gradient Elution Conditions;1.7. Influence of the Test Conditions on the Obtained Kinetic-Performance Limit Curve;1.8. Some Reflections on Recent Trends in Liquid Chromatography using the Kinetic Plot Method;1.9. Conclusions;Acknowledgements;References;2.1 UHPLC Instrumentation;2.2 UHPLC Columns;3.1 Introduction;3.2 Qualitative Transfer from HPLC to UHPLC;3.3 Normative Context for the HPLC to UHPLC Transfer;3.4 Validation of UHPLC Methods and Equivalence of the HPLC-UHPLC Methods;3.5 Conclusions;3.6 References;4.1 Introduction;4.2 High throughput and high resolution in HT-UHPLC;4.3 Limitations of HTLC and HT-UHPLC;4.4 Advantage of high temperature in life science analysis;4.5 HT-UHPLC in comprehensive on-line two-dimensional liquid chromatography (LC x LC);4.6 Conclusion;5. Comparison of the performance of totally porous and core-shell particles;5.1. Introduction;5.2. Column performance;5.3. Possibilities of recent core-shell technology;5.4. Particle size distribution and roughness of core-shell particles;5.5. Loading capacity of core-shell particles;5.6. Limited efficiency when core-shell particles packed in narrow-bore columns;5.7. Extra column effects, contribution to band broadening;5.8. Performance of core-shell and totally porous particles in isocratic elution mode;5.9. Performance of core-shell and totally porous particles in gradient elution mode;5.10. Conclusion;6.1 Introduction;6.2 Analytical Conditions for performing HILIC.;6.3 Applications of HILIC in UHPLC.;6.4 References.;7.1 Introduction;7.2 Selection of ionization techniques;7.3 Overview of mass analyzers and their main features;7.4 New developments in mass spectrometry applicable in UHPLC/MS;7.5 Conclusions;8.1 Introduction;8.2 Solubility;8.3 Ionization;8.4 Lipophilicity;8.5 Permeability;8.6 Conclusion;8.7 References;9.1 Introduction;9.2 UHPLC in Bioanalysis;9.3 Sample preparation for UHPLC in bioanalysis;9.4 Conclusions;10.1 Introduction;10.2 GC-MS Analyses;10.3 LC-MS(/MS) Analyses;10.4 Application of UHPLC-MS(/MS) for Drug Testing in Sports;10.5 Conclusion;References;11.1 Introduction;11.2 Use of UHPLC for the Analysis of Seized Drugs;12.1. Pharmaceuticals as environmental contaminants;12.2. Analysis of pharmaceuticals in environmental samples;12.3. Occurrence of pharmaceuticals in environmental and wastewater samples;12.4. Conclusions;Acknowledgements;References;13.1 Introduction;13.2 Multiple Facets of UHPLC in NP research;13.3 Fast Targeted Analysis;13.4 Fast Non-Targeted Analysis, Fingerprinting, and Metabolomics;13.5 High-Resolution Profiling and Metabolite ID;13.6 Conclusion;Acknowledgments;References;14.1. Introduction;14.2. Pre-analysis Considerations: Protocol Design, Sample Collection, Storage and Preparation;14.3. Sample Preparation for Serum and/Plasma;14.4. Collection and Storage of Urine Samples;14.5. UHPLC-MS-Based Metabolite Profiling;14.6. Applications of UHPLC-MS to Human Metabolic Profiling Studies;14.7 Current Challenges for UHPLC-MS in Global metabolic profiling Studies;14.8. Conclusions