Proteomics

Methods Express

AvC. David O'Connor,B. David Hames

Inbunden, Engelska, 2007

Del i serien Methods Express (Hardcover)

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Beskrivning

Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation.The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house.Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.

Produktinformation

Utgivningsdatum:2007-11-01
Mått:180 x 250 x 20 mm
Vikt:805 g
Format:Inbunden
Språk:Engelska
Serie:Methods Express (Hardcover)
Antal sidor:300
Förlag:Scion Publishing Ltd
ISBN:9781904842200

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

1. Sample preparation and subcellular fractionation approaches: purification of membranes and their microdomains for mass spectrometry analysisYan Li , Phil Oh, and Jan E. Schnitzer, Sidney Kimmel Cancer Center2. An isotope-coding strategy for quantitative proteomicsXian Chen, Dept of Biochemistry and Biophysics, University of N. Carolina at Chapel Hill3. Gel-based approachesStuart Cordwell and Ben Crossett, both at School of Molecular and Microbial Biosciences, University of Sydney, and Melanie Y. White, Minomic Pty Ltd4. Peptide sorting by reverse-phase diagonal chromatographyKris Gevaert and Joel Vandekerckhove, Faculty of Medicine and Health Sciences, Ghent University5. Mass spectrometry strategies for protein identificationDavid R. Goodlett, University of Washington and Garry L. Corthals, University of Turku6. Desorption electrospray ionization: proteomics studies by a method that bridges ESI and MALDIZoltan Takats, Justin M. Wiseman, Demian R. Ifa and R.Graham Cooks, all at Dept of Chemistry, Purdue University7. Analysis of cellular protein complexes by affinity purification and mass spectrometryTilmann Buerckstuemmer and Keiryn L. Bennett, both at Research Center for Molecular Medicine of the Austrian Academy of Sciences8. Clinical proteomic profiling and disease signaturesRosamonde E. Banks, David A. Cairns, David N. Perkins and Jennifer H. Barrett, all at Cancer Research UK Clinical Centre, St James' s University Hospital, Leeds9. Characterization of post-translational modifications: undertaking the phosphoproteomeW. Andy Tao, Purdue University; Bernd Bodenmiller and Ruedi Aebersold, both Institute for Molecular Systems Biology, Federal Institute of Technology, Zurich10. Protein microarray technologiesChien-Sheng Chen, Sheng-Ce Tao, and Heng Zhu, Dept of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine11. Intelligent mining of complex data: challenging the proteomic bottleneckDan Bach Kristensen, Maxygen and Alexandre Potelejnikov, Proxeon12. Bioinformatic approaches in proteomicsSandra Orchard and Henning Hermjakob, both European Bioinformatics Institute, HinxtonList of suppliersIndex