Oxygen Isotopes of Inorganic Phosphate in Environmental Samples

Joseph Adu-Gyamfi, Soil and Water Management and Crop Nutrition (SWMCN) Section, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency (IAEA), Vienna, AustriaHe is working as Integrated Soil Fertility Management Officer providing technical assistance to IAEA national, regional/interregional technical cooperation projects in in Africa, Asia, Europe, Latin America, and the Caribbean, on the use of multi-stable isotope fingerprints to identify sources and transport of contaminants in the environment.  His work involved developing climate-smart agricultural practices to combat and mitigate the impacts of climate change on agricultural systems and food security, soil and water quality in developing countries. Prior to joining the SWMCN Section, he has 30 years’ experience in the use of isotopes to measure and monitor interactions between soil, water, and nutrients in cropping systems at different research, academic, development consultant firms, national and international organizations in both developing and developed countries. He is Author and Coauthor of 3 books, several chapters, and research publications in refereed journals. He has won several awards for his professional achievements including the ICRISAT Outstanding Research Scientist 2002, special award on Root Research on Drylands (Japanese Society of Root Research), IAEA Superior Achievement Award in 2018. Verena Pfahler, Smithsonian Tropical Research Institute, Balboa, Ancon, Panama.  Her area of expertise includes phosphorus dynamics and impacts in the environment and the use of stable and radioisotopes in environmental studies. She is Postdoctoral Research Fellow focusing on phosphorus cycling in the soil/plant system and P cycling in agricultural systems, alternative P fertilizers, and using oxygen isotopes associated with P (δ18OP) to study P cycling. She has over ten years of experience in using the δ18Op protocol and adapting it to different soil and plant extracts and is Coauthor of one chapter and 13 peer review journal papers. Her recent work includes P cycling in tropical rainforests using the δ18OP method.

• Chapter 1. The Use of the δ18OP to Study P Cycling in the Environment.- Chapter 2. Extraction Protocol.- Chapter 3. Purification Protocol.- Chapter 4. Modifications and Issues during Purification.- Chapter 5. How to Design a Study including the Analysis of δ18OP.- Chapter 6. Conclusions and Way Forward.